LOCUS       sequence-138922-        7667 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sema6A, isoform a protein,
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7667)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7667)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema6a.a-Fc-His
FEATURES             Location/Qualifiers
     source          1..7667
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4247..4264
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4290..4411
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4327..4346)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4381..4400
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4449..4466)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4620..5208)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4700..4719)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5379..6170,6171..6239))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6002..6021
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6240..6344)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6412..6430
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6468..6490)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6492..6512)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6497..6827
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6678..6813
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6740..6759
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6964..6983
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7050..7505)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7187..7208)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7399..7418
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7631..7653
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7645..7662
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7646..7662
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgcggccagc
     1621 agccttactg ctgtgtctca cactgctaca ctgcgctggg gctggtttcc cagaagattc
     1681 cgagccaatc agtatttcgc atggcaacta tacaaaacag tatccggtgt ttgtgggcca
     1741 caagccagga cggaacacca cgcagaggca caggctggac atccagatga tcatgatcat
     1801 gaacagaacc ctctacgttg ctgctcgaga ccatatttat actgttgata tagacacatc
     1861 ccacacagaa gaaatttact gtagcaaaaa actgacatgg aaatctagac aggctgacgt
     1921 agacacatgc aggatgaagg ggaaacataa ggatgaatgt cacaacttca ttaaagttct
     1981 tctcaagaag aatgatgata cgctgtttgt ctgtggaacc aatgccttca acccttcctg
     2041 cagaaactac agggtcgata ccttggaaac ttttggggat gaatttagcg gaatggccag
     2101 atgcccttat gatgccaaac atgccaacat cgctctgttt gcagatggaa aactctactc
     2161 ggctacagtg actgactttc tggccattga tgcggtcatt tacaggagcc tcggagacag
     2221 ccctaccctc aggactgtca agcatgattc aaagtggttg aaagagccgt actttgtcca
     2281 agccgtggat tatggggact atatctactt cttcttcaga gaaattgcag tagaatacaa
     2341 cactatgggg aaggttgttt tccctagggt ggctcaggtc tgtaagaatg acatgggagg
     2401 gtctcagaga gtcctggaga agcagtggac atctttcctg aaggctcgcc tgaactgctc
     2461 ggtgcctgga gactctcatt tttatttcaa tatactccag gcagttacag atgtgattcg
     2521 cattaatggc cgtgatgttg tcttggcaac cttttccaca ccttataaca gcatcccagg
     2581 ttctgcagtc tgtgcctatg acatgcttga cattgctaat gttttcactg ggaggttcaa
     2641 ggaacagaaa tcacctgact ctacctggac acccgttcca gacgaacgag tccctaagcc
     2701 caggccaggc tgttgtgctg gatcatcctc tttagaaaaa tatgcaacct ccaatgagtt
     2761 tcccgatgat accctgaact tcattaagac gcatccactc atggacgagg cagtgccttc
     2821 catcatcaac agaccttggt tcctgagaac aatggtcaga taccgcctga ccaaaattgc
     2881 agtagacaac gctgccgggc catatcagaa tcacactgtg gttttcctgg gatcagaaaa
     2941 gggaatcatc ctgaagttct tggccaggat aggaagcagt ggtttcctaa atggcagcct
     3001 tttcctggag gagatgaatg tttacaaccc agaaaagtgc agctatgatg gtgtagaaga
     3061 caaaaggatc atgggcatgc agctcgacag agcgagtggc tcactctatg ttgcattctc
     3121 tacttgtgtg atcaaggtgc ctcttggccg ctgtgagcga catgggaagt gtaaaaaaac
     3181 ctgcatcgcc tccagagacc cgtattgtgg gtgggtaagg gaaagtggtt cctgtgccca
     3241 tctgtcaccc cttagcagac tgacatttga gcaggacatt gagcgtggca atacggacgg
     3301 cctaggagac tgtcacaatt ccttcgtggc actgaatggg cacgccagtt ccctctatcc
     3361 cagcaccact acgtcagatt cggcatcccg agacgggtat gagtctaggg gaggcatgct
     3421 ggactggaac gacctgctcg aggcacctgg cagcacagac cctttggggg cagtgtcctc
     3481 tcataaccac caggacaaga agggagtgat tcgggaaagt tacctcaaaa gcaacgacca
     3541 gcttgttcct ggcgcgcctg acaaaactca cacatgccca ccgtgcccag cacctgaact
     3601 cctgggggga ccgtcagtct tcctcttccc cccaaaaccc aaggacaccc tcatgatctc
     3661 ccggacccct gaggtcacat gcgtggtggt ggacgtgagc cacgaagacc ctgaggtcaa
     3721 gttcaactgg tacgtggacg gcgtggaggt gcataatgcc aagacaaagc cgcgggagga
     3781 gcagtacaac agcacgtacc gtgtggtcag cgtcctcacc gtcctgcacc aggactggct
     3841 gaatggcaag gagtacaagt gcaaggtctc caacaaagcc ctcccagccc ccatcgagaa
     3901 aaccatctcc aaagccaaag ggcagccccg agaaccacag gtgtataccc tgcccccatc
     3961 ccgggatgag ctgaccaaga accaggtcag cctgacctgc ctggtcaaag gcttctatcc
     4021 cagcgacatc gccgtggagt gggagagcaa tgggcagccg gagaacaact acaagaccac
     4081 gcctcccgtg ctggactccg acggctcctt cttcctctac agcaagctca ccgtggacaa
     4141 gagcaggtgg cagcagggga acgtcttctc atgctccgtg atgcatgagg ctctgcacaa
     4201 ccactacacg cagaagagcc tctccctgtc tccgggtaaa ggcggccatc atcaccatca
     4261 ccattaatct agagatctta ttaaagcaga acttgtttat tgcagcttat aatggttaca
     4321 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     4381 gtggtttgtc caaactcatc aatgtatctt atcatgtctg gtcgactcta gctagactct
     4441 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     4501 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     4561 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     4621 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4681 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4741 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4801 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4861 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4921 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4981 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5041 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     5101 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     5161 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     5221 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     5281 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     5341 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     5401 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     5461 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     5521 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     5581 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     5641 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5701 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5761 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5821 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5881 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5941 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6001 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6061 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     6121 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     6181 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     6241 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     6301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     6361 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt
     6421 atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     6481 cagctcccgg aggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccaggcag
     6541 gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag
     6601 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc
     6661 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     6721 atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat
     6781 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag
     6841 cttgtatatc cattttcgtc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     6901 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     6961 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     7021 gatgcgtaag gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt
     7081 cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat
     7141 cccttataaa tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa
     7201 gagtccacta ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg
     7261 cgatggccca ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa
     7321 agcactaaat cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc
     7381 gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag
     7441 tgtagcggtc acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg
     7501 cgcgtccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     7561 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     7621 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgaatt
//
