LOCUS       sequence-138937-        7705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sema4F, isoform a protein,
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7705)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema4f.a-Fc-His
FEATURES             Location/Qualifiers
     source          1..7705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             4285..4302
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4328..4449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4365..4384)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4419..4438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4487..4504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4658..5246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4738..4757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5417..6208,6209..6277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6040..6059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6278..6382)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6450..6468
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6506..6528)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6530..6550)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6535..6865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6716..6851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6778..6797
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7002..7021
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7088..7543)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7225..7246)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7437..7456
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7669..7691
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7683..7700
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7684..7700
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gccaccatgc tggccagggc cgagcggccc
     1621 cgcccgggcc cccggccgcc tccggtctct ctcttcccgc cgccgtcgtc gctgctgctg
     1681 ctgctgctgg cgatgctgag cgccccggtg tgcggccgcg tcccccgctc ggtgcccaga
     1741 acctcgctgc ccatctccga ggctgactcc tatctcaccc ggtttgcggc ccctcatacg
     1801 tacaattact ctgctctcct ggtggatcct gcttctcaca cactttacgt cggtgcacgg
     1861 gacagcatct tcgctttaac cctgcccttc tctggggaaa agcctcgaag gatcgactgg
     1921 atggtgcccg agactcacag acagaactgt aggaagaaag gcaagaaaga ggacgaatgt
     1981 cacaatttta tccagattct cgccattgcc aatgcctctc acctcctcac gtgtggcacc
     2041 ttcgcttttg atccgaagtg cggggttatt gatgtgtcca gtttccagca ggttgaaaga
     2101 cttgagagtg gccgggggaa atgtcctttt gagccagctc aacggtcagc agctgtaatg
     2161 gctgggggcg tcctctacac cgccactgtg aaaaacttcc tggggacaga gccgattatc
     2221 tcccgagctg tgggtcgagc tgaggactgg attcgaacag agaccttgtc atcctggctt
     2281 aatgccccag cctttgtcgc agctatggtc ctgagcccgg ctgagtgggg ggatgaagat
     2341 ggagacgatg aaatcttctt tttcttcacg gagacctccc gagtcttgga ctcatatgag
     2401 cgcatcaagg tcccacgagt ggcccgtgtg tgtgcggggg accttggggg ccggaagacc
     2461 cttcagcaga gatggacgac gtttctaaag gctgacctgc tgtgtccagg gcccgagcat
     2521 ggaagggcct cgggggttct gcaggatatg acagagcttc gacctcagcc tggcgcgggg
     2581 acccccctct tttatggcat cttttcctcc cagtgggaag gagccgccat ttctgctgtg
     2641 tgtgccttcc gaccccaaga catccgggca gtgctgaatg gtccctttag agagctaaaa
     2701 catgactgca acaggggact acctgtcatg gacaacgagg tgccccagcc cagacctgga
     2761 gagtgcatca ccaacaacat gaagttccag cagtttggat cctcactctc cctgccagac
     2821 cgtgtgctca cctttatcag agaccacccg ctcatggaca ggcccgtgtt tccagctgat
     2881 ggccgccccc tgctggtcac tacggataca gcctatctca gagttgtggc tcaccgggtg
     2941 accagcctct cagggaaaga atatgatgtg ctctacctag ggacagagga tgggcacctc
     3001 caccgggctg tgcgcatcgg agctcagctc agtgtcctgg aggatctggc cttgttcccg
     3061 gaaccacaac cggttgagag catgaaattg taccatgatt ggctcctggt gggctcccat
     3121 actgaggtga cacaagtgaa caccagcaac tgtggccgtc tccagagctg ctcagaatgt
     3181 atcctggccc aggatcccgt gtgtgcctgg agcttccggc tcgatgcttg tgtggcccat
     3241 gcaggcgagc accgtgggat ggttcaagac atagagtcag cagatgtctc ttctttgtgt
     3301 ccaaaagaac ctggagaaca tccggtagtg tttgaagttc ctgtggctac ggtgggccac
     3361 gtggtcctgc cgtgctcccc cagttctgcc tgggcatcct gtgtgtggca ccagcccagt
     3421 ggagtgactt cgctcacccc ccgaagggat ggactagagg tggtggtgac cccaggggcc
     3481 atgggggctt atgcttgcga gtgtcaggag ggtggagccg cccgtgtggt ggctgcttat
     3541 agcttggtgt ggggcagcca gagggggccc tcaaaccggg cccacaccgg cgcgcctgac
     3601 aaaactcaca catgcccacc gtgcccagca cctgaactcc tggggggacc gtcagtcttc
     3661 ctcttccccc caaaacccaa ggacaccctc atgatctccc ggacccctga ggtcacatgc
     3721 gtggtggtgg acgtgagcca cgaagaccct gaggtcaagt tcaactggta cgtggacggc
     3781 gtggaggtgc ataatgccaa gacaaagccg cgggaggagc agtacaacag cacgtaccgt
     3841 gtggtcagcg tcctcaccgt cctgcaccag gactggctga atggcaagga gtacaagtgc
     3901 aaggtctcca acaaagccct cccagccccc atcgagaaaa ccatctccaa agccaaaggg
     3961 cagccccgag aaccacaggt gtataccctg cccccatccc gggatgagct gaccaagaac
     4021 caggtcagcc tgacctgcct ggtcaaaggc ttctatccca gcgacatcgc cgtggagtgg
     4081 gagagcaatg ggcagccgga gaacaactac aagaccacgc ctcccgtgct ggactccgac
     4141 ggctccttct tcctctacag caagctcacc gtggacaaga gcaggtggca gcaggggaac
     4201 gtcttctcat gctccgtgat gcatgaggct ctgcacaacc actacacgca gaagagcctc
     4261 tccctgtctc cgggtaaagg cggccatcat caccatcacc attaatctag agatcttatt
     4321 aaagcagaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     4381 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     4441 tgtatcttat catgtctggt cgactctagc tagactcttc cgcttcctcg ctcactgact
     4501 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     4561 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     4621 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     4681 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     4741 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     4801 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     4861 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     4921 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     4981 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     5041 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     5101 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     5161 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     5221 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     5281 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     5341 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     5401 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     5461 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     5521 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     5581 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     5641 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     5701 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     5761 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     5821 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     5881 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     5941 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     6001 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     6061 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     6121 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     6181 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     6241 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     6301 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     6361 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
     6421 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg
     6481 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag gaatgtgtgt
     6541 cagttagggt gtggaaagtc cccaggctcc ccaggcaggc agaagtatgc aaagcatgca
     6601 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6661 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6721 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6781 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
     6841 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcgtccc
     6901 ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc
     6961 gtcagcgggt gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt
     7021 actgagagtg caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7081 catcaggaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     7141 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     7201 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     7261 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     7321 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     7381 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     7441 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     7501 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtccattcg ccattcaggc
     7561 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     7621 aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     7681 gttgtaaaac gacggccagt gaatt
//
