LOCUS       sequence-139042-        9308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector encoding centrin-1 with N-terminal FLAG and 
            mCherry tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9308)
  TITLE     pLVX-EGFP-C3-Cep123
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLVX-FLAG-mCherry-C1-centrin-1
FEATURES             Location/Qualifiers
     source          1..9308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             4..27
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             28..732
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGT
                     QTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFE
                     DGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKG
                     EIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAE
                     GRHSTGGMDELYK"
     primer_bind     complement(170..188)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     438..457
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        1399..1898
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1434..1457)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     1831..1850
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             1919..2518
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1919..1938)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2415..2435
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2532..3120
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2585..2605)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3003..3014)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3327..3960
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4088..4104)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4088..4104)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4101..4123)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4112..4128
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4136..4142,4143..4160,4161..4166))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4181..4202
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4319..4336)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4490..5078)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4570..4589)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5249..6040,6041..6109))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5872..5891
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6110..6214)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6262..6396
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6299..6318)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6353..6372
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6564..7197
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7244..7369
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7866..8099
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8284..8328
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8591..8706
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     enhancer        8764..9067
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9068..9271
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9222..9242
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atggattaca aggatgacga cgataaggtg agcaagggcg aggaggataa catggccatc
       61 atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg ccacgagttc
      121 gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag
      181 gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca gttcatgtac
      241 ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa gctgtccttc
      301 cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg
      361 acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct gcgcggcacc
      421 aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga ggcctcctcc
      481 gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag gctgaagctg
      541 aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa gaagcccgtg
      601 cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca caacgaggac
      661 tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg cggcatggac
      721 gagctgtaca agtccggact cagatctcga gctcaagctt cgaattctgc agtcgacggt
      781 accatggctt ccggcttcaa gaagcccagc gctgcctcca ccggccaaaa gagaaaggtg
      841 gcacctaagc ccgagctcac tgaggatcag aagcaagaag ttcgggaagc atttgacctc
      901 ttcgacgtgg acggaagtgg gaccatcgac gcgaaggagc tgaaggtggc catgagagcg
      961 ctgggcttcg aacccaggaa ggaagagatg aagaaaatga tctccgaggt ggacagggaa
     1021 ggcacgggga agatcagctt caatgacttc ctggccgtga tgacgcagaa gatgtccgag
     1081 aaggacacca aagaagaaat cctgaaggcc ttcaggctct ttgatgacga tgagaccggg
     1141 aagatctcgt tcaaaaacct gaagcgtgtg gccaacgagc tgggggagaa cctcacggat
     1201 gaggagctgc aggagatgat cgacgaagct gatcgggatg gggacggcga agtgaacgag
     1261 gaggagttcc ttcggatcat gaagaagacc agcctttact gaggatccgc ggccgcgacc
     1321 ctaggccgac gcgtccggtt aaccgggatc gagatcctgc agaaccacga gtctggttgc
     1381 attctagata attctaccgg gtaggggagg cgcttttccc aaggcagtct ggagcatgcg
     1441 ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct cgcacacatt
     1501 ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc gcgccacctt
     1561 ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt
     1621 gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc gctgagcaat
     1681 ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc gctttctggg
     1741 ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc aggggcgggg
     1801 cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg
     1861 ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca agcttaccat
     1921 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
     1981 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
     2041 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
     2101 cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg gtctggacca cgccggagag
     2161 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
     2221 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
     2281 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
     2341 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
     2401 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
     2461 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacc
     2521 gcgtctggaa caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     2581 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     2641 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     2701 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     2761 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2821 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2881 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     2941 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     3001 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     3061 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     3121 ctggaattaa ttctgcagtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
     3181 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
     3241 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
     3301 ccacttttta aaagaaaaga ggggactgga agggctaatt cactcccaac gaagacaaga
     3361 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
     3421 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
     3481 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
     3541 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
     3601 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
     3661 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
     3721 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
     3781 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     3841 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     3901 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     3961 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4021 agagtgagag gccttgacat tgctagcgtt taccgtcgac ctctagctag agcttggcgt
     4081 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     4141 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     4201 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4261 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     4321 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     4381 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     4441 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     4501 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     4561 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     4621 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     4681 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     4741 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     4801 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     4861 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     4921 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     4981 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     5041 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     5101 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     5161 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     5221 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     5281 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     5341 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     5401 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     5461 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     5521 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     5581 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     5641 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     5701 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     5761 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     5821 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     5881 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     5941 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     6001 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     6061 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     6121 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     6181 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     6241 acgtcgacgg atcgggagat caacttgttt attgcagctt ataatggtta caaataaagc
     6301 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     6361 tccaaactca tcaatgtatc ttatcatgtc tggatcaact ggataactca agctaaccaa
     6421 aatcatccca aacttcccac cccataccct attaccactg ccaattacct gtggtttcat
     6481 ttactctaaa cctgtgattc ctctgaatta ttttcatttt aaagaaattg tatttgttaa
     6541 atatgtacta caaacttagt agttggaagg gctaattcac tcccaaagaa gacaagatat
     6601 ccttgatctg tggatctacc acacacaagg ctacttccct gattagcaga actacacacc
     6661 agggccaggg gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     6721 gccagataag gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag
     6781 cctgcatggg atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct
     6841 agcatttcat cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg
     6901 agcttgctac aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac
     6961 tggggagtgg cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt
     7021 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     7081 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     7141 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     7201 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     7261 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     7321 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     7381 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     7441 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     7501 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     7561 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     7621 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     7681 caaaagtaag accaccgcac agcaagcggc cggccgctga tcttcagacc tggaggagga
     7741 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     7801 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     7861 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     7921 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     7981 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     8041 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     8101 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     8161 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     8221 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     8281 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     8341 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     8401 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     8461 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     8521 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     8581 cggtatcgcc tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     8641 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     8701 aaattttcgg gtttattaca gggacagcag agatccagtt tatcgataag cttgggagtt
     8761 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     8821 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     8881 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     8941 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     9001 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     9061 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     9121 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     9181 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     9241 tgtacggtgg gaggtctata taagcagagc tggtttagtg aaccgtcaga tccgctagcg
     9301 ctaccggt
//
