LOCUS       sequence-139062-        9263 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 9263)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-PhCHS-CmCHI
FEATURES             Location/Qualifiers
     source          1..9263
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    3..27
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             42..64
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             1728..1772
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      1825..1872
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        1906..1924
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    1925..1949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             1964..1986
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             3185..3229
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      3282..3329
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     promoter        3363..3381
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    3382..3406
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             3421..3443
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4198..4242
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     terminator      4295..4342
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      4386..4841
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     4683..4704
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     CDS             complement(join(4956..5747,5748..5816))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5579..5598
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5817..5909)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5990..6578
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6479..6498
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6732..6749
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    6764..6906
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(6832..6851)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6992..7014
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     CDS             complement(7008..7199)
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     CDS             complement(7771..8853)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     8815..8834
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(8854..8931)
                     /gene="lacI"
                     /label=lacI promoter
     primer_bind     9137..9156
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        join(9247..9263,1..2)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggggaattgt gagcggataa caattcccct ctagaaataa ttttgtttaa ctttaagaag
       61 gagatataca tatgggagac tgtgtagcac ccaaagaaga ccttattttc cgatcgaaac
      121 tccctgatat ttacatcccg aaacaccttc cgttacatac ttattgtttc gaaaacatct
      181 cgaaagttgg cgacaagtcc tgtttaataa atggcgctac aggcgaaacg ttcacttatt
      241 cccaagttga gctcctttcc aggaaagttg catcagggtt aaacaaactc ggcattcaac
      301 agggcgatac catcatgctt ttgctcccta actcccctga gtattttttc gctttcttag
      361 gcgcatcgta tcgtggtgca atttctacta tggccaatcc gtttttcact tctgctgagg
      421 tgatcaaaca gctcaaagca tcccaagcta agctcataat tacgcaagct tgttacgtag
      481 acaaagtgaa agactacgca gcagagaaaa atatacagat catttgcatc gatgatgctc
      541 ctcaggattg tttacatttc tccaaactta tggaagctga tgaatcagaa atgcctgagg
      601 ttgtgatcaa ttcagacgat gtcgtcgcgt taccttactc atcgggtact acaggactac
      661 cgaaaggtgt tatgttgaca cacaaaggac ttgttactag cgtggcacaa caagttgatg
      721 gagacaatcc gaatttatat atgcatagcg aggatgtgat gatctgcata ttgcctttgt
      781 ttcatattta ttcgcttaac gcggtgttgt gctgtggact cagagcaggg gtgacgatct
      841 tgattatgca gaaatttgat attgtgccat ttttggaact gatacagaaa tataaagtta
      901 caattggacc gtttgtgcca ccaattgtgt tggcaattgc gaaaagtcca gtggtggata
      961 aatatgactt gtcgtcggtg aggacggtta tgtctggagc tgctccgtta gggaaggagc
     1021 ttgaagatgc tgttagagct aagtttccta atgccaaact tggtcaggga tatggaatga
     1081 cagaggcagg gccagtttta gcaatgtgcc tggcgtttgc aaaggaacca tacgagatca
     1141 aatcgggtgc ctgtggaact gttgtgagga atgctgaaat gaaaattgtg gatcctgaga
     1201 ccaacgcctc tcttccacga aaccaacgcg gagagatttg cattcgaggt gaccaaatta
     1261 tgaaaggcta cctcaatgat cctgaatcaa caaggacaac aatagacgaa gaaggctggt
     1321 tgcacacagg agatataggc ttcattgacg acgatgatga gctatttatt gttgatagac
     1381 ttaaggaaat aatcaaatac aaaggcttcc aggttgcccc tgctgaactt gaagctctgc
     1441 tacttactca tcctaccatt tccgatgctg cagttgttcc catgatagat gagaaagcag
     1501 gagaggtgcc tgtggctttt gttgtgagaa caaacggttt caccaccact gaggaagaaa
     1561 tcaagcaatt cgtctcgaaa caggtggtgt tctacaagag aatatttcgt gtattttttg
     1621 ttgatgcaat tccgaaatca ccatctggaa agattcttcg aaaggacttg agagcaaaaa
     1681 tagcatccgg tgatcttccc aaataatagg gtaccctcga gtctggtaaa gaaaccgctg
     1741 ctgcgaaatt tgaacgccag cacatggact cgtctactag tcgcagctta attaacctaa
     1801 actgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
     1861 aggggttttt tgctaggatc gagatcgatc tcgatcccgc gaaattaata cgactcacta
     1921 taggggaatt gtgagcggat aacaattccc ctctagaaat aattttgttt aactttaaga
     1981 aggagatata catatggtga cagtcgagga gtatcgtaag gcacaacgtg ctgaaggtcc
     2041 agccactgtc atggccattg gaacagccac accttcaaac tgtgttgatc aaagcactta
     2101 ccctgatttt tattttcgta tcactaacag tgagcacaag actgatctta aggagaaatt
     2161 taagcgcatg tgtgaaaaat caatgattaa gaaaaggtac atgcacttaa cagaggaaat
     2221 cttgaaagag aatcctagta tgtgtgaata catggcacct tctcttgatg ctaggcaaga
     2281 catagtggtg gttgaagtgc ccaaacttgg caaagaggca gctcaaaagg ccatcaagga
     2341 atggggccag cccaagtcca aaattaccca tttggtcttt tgcacaacca gtggtgtgga
     2401 catgcctggg tgtgactatc aactcactaa gctacttggg cttcgtccat cggtcaagag
     2461 gcttatgatg taccaacaag gttgctttgc tggtggcacg gttcttcggt tagccaagga
     2521 cttggctgaa aacaacaagg gcgctcgagt ccttgttgtt tgttcagaaa tcaccgcggt
     2581 cactttccgt gggccaaatg atactcattt ggatagttta gttggccaag cactttttgg
     2641 tgatggggca ggcgcgatca ttataggttc tgatccaatt ccaggggtcg aaaggccttt
     2701 gttcgagctc gtttcagcag cccaaactct tctcccagat agccatggtg ctattgatgg
     2761 ccatctccgt gaagttgggc ttacattcca cttactcaaa gatgttcctg ggctgatctc
     2821 aaaaaatatt gagaagagcc ttgaggaagc attcaaacct ttgggcattt ctgattggaa
     2881 ctctctattc tggattgctc atccaggtgg gcctgcaatt ttggaccaag ttgaaataaa
     2941 gttgggccta aagcccgaga aacttaaggc tacaaggaat gtgttaagta actatggtaa
     3001 catgtcaagt gcttgtgtac tgtttatttt ggatgaaatg agaaaggcct cagccaaaga
     3061 aggtttagga actactggtg aagggcttga gtggggtgtt ctttttggat ttgggcctgg
     3121 gctaacagtt gagactgttg tcctccacag tgttgctact taatagggta ccctcgagtc
     3181 tggtaaagaa accgctgctg cgaaatttga acgccagcac atggactcgt ctactagtcg
     3241 cagcttaatt aacctaaact gctgccaccg ctgagcaata actagcataa ccccttgggg
     3301 cctctaaacg ggtcttgagg ggttttttgc taggatcgag atcgatctcg atcccgcgaa
     3361 attaatacga ctcactatag gggaattgtg agcggataac aattcccctc tagaaataat
     3421 tttgtttaac tttaagaagg agatatacat atgaatccgt cgccgtctgt taccgaactg
     3481 caagtggaaa atgtcacctt tacgccgagt ctgcaaccgc cgggctctac caaatcgcat
     3541 tttctgggcg gtgcaggtga acgtggcctg gaaatcgaag gcaaatttgt taaattcacc
     3601 gctattggtg tctatctgga agaaaacgcc gtgccgctgc tggcaggcaa atggaaaggc
     3661 aaaaccgccg gtgaactgac ggaatctgtc gaatttttcc gcgatgtggt taccggcccg
     3721 tttgaaaaat tcatgaaagt gaccatgatc ctgccgctga cgggtgcgca gtattcagaa
     3781 aaagttgctg aaaattgcat ggcgatttgg aaatttttcg gcatctacac cgatgcagaa
     3841 gctaaagcga ttgaaaaatt tacggaagtg ttcaaagacg aaatttttcc gccgggcagc
     3901 tctatcctgt tcacccaaag ttccggttcg ctgacgattt cattttcgaa agatggcagc
     3961 atcccgaaag acggtgtcgc ggtgattgaa aacaatctgc tgagcgaagc cgttctggaa
     4021 tctatgatcg gtaaaaacgg cgtcagtccg gcggccaaaa aatccctggc cgaacgtctg
     4081 tcagcactgc tgaatgttgc ttccgacaaa atgaaaggcg gtggcggctc aggtggcggt
     4141 ggctctggtg gcggtggttc aggcgtcaaa gaaagtctgg tgtgactcga gtctggtaaa
     4201 gaaaccgctg ctgcgaaatt tgaacgccag cacatggact cgtctactag tcgcagctta
     4261 attaacctaa actgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa
     4321 acgggtcttg aggggttttt tgctagcgaa aggaggagtc gactatatcc ggattggcga
     4381 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4441 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     4501 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4561 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     4621 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4681 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     4741 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     4801 atttaacgcg aattttaaca aaatattaac gtttacaatt tctggcggca cgatggcatg
     4861 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     4921 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     4981 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     5041 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     5101 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     5161 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     5221 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     5281 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     5341 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     5401 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     5461 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     5521 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     5581 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     5641 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     5701 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     5761 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     5821 ttcctttttc aatcatgatt gaagcattta tcagggttat tgtctcatga gcggatacat
     5881 atttgaatgt atttagaaaa ataaacaaat aggtcatgac caaaatccct taacgtgagt
     5941 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     6001 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     6061 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     6121 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     6181 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     6241 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     6301 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6361 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6421 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6481 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6541 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6601 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     6661 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     6721 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc
     6781 tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg
     6841 ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg
     6901 gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     6961 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     7021 ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc
     7081 gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt
     7141 aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact
     7201 gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag
     7261 aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag
     7321 ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc
     7381 cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg
     7441 cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca
     7501 cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg
     7561 cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag
     7621 cctagccggg tcctcaacga caggagcacg atcatgctag tcatgccccg cgcccaccgg
     7681 aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg tgcctaatga
     7741 gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     7801 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     7861 cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc ccttcaccgc
     7921 ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca ggcgaaaatc
     7981 ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat cgtcgtatcc
     8041 cactaccgag atgtccgcac caacgcgcag cccggactcg gtaatggcgc gcattgcgcc
     8101 cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct cattcagcat
     8161 ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt ccgctatcgg
     8221 ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac gcgccgagac
     8281 agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga ccagatgctc
     8341 cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg gtgtctggtc
     8401 agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag caatggcatc
     8461 ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga gaagattgtg
     8521 caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca ccacgctggc
     8581 acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg cgtgcagggc
     8641 cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt gttgtgccac
     8701 gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc gcgttttcgc
     8761 agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga caccggcata
     8821 ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt gactctcttc
     8881 cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt ccgggatctc
     8941 gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt
     9001 tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg
     9061 ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag
     9121 cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc
     9181 cggtgatgcc ggccacgatg cgtccggcgt agcctaggat cgagatcgat ctcgatcccg
     9241 cgaaattaat acgactcact ata
//
