LOCUS       sequence-139063-         845 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses three enzymes to convert phenylpropanoic acids to 
            flavanones in vivo.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 845)
  AUTHORS   Jones JA, Vernacchio VR, Sinkoe AL, Collins SM, Ibrahim MH, Lachance
            DM, Hahn J, Koffas MA
  TITLE     Experimental and computational optimization of an Escherichia coli 
            co-culture for the efficient production of flavonoids.
  JOURNAL   Metab Eng. 2016 Feb 6;35:55-63. doi: 10.1016/j.ymben.2016.01.006.
  PUBMED    26860871
REFERENCE   2  (bases 1 to 845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab 
            Eng."; date: "2016-02-6"; volume: "35:55-63. doi"; pages: " 
            10.1016/j.ymben.2016.01.006"
COMMENT     Sequence Label: pETM6-Pc4CL-CmCHS-MsCHI
FEATURES             Location/Qualifiers
     source          1..845
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     52..71
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        52..70
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    71..95
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             110..132
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 cgatgcgtcc ggcgtagcct aggatcgaga tcgatctcga tcccgcgaaa ttaatacgac
       61 tcactatagg ggaattgtga gcggataaca attcccctct agaaataatt ttgtttaact
      121 ttaagaagga gatatacata tgggagactg tgtagcaccc aaagaagacc ttattttccg
      181 atcgaaactc cctgatattt acatcccgaa acaccttccg ttacatactt attgtttcga
      241 aaacatctcg aaagttggcg acaagtcctg tttaataaat ggcgctacag gcgaaacgtt
      301 cacttattcc caagttgagc tcctttccag gaaagttgca tcagggttaa acaaactcgg
      361 cattcaacag ggcgatacca tcatgctttt gctccctaac tcccctgagt attttttcgc
      421 tttcttaggc gcatcgtatc gtggtgcaat ttctactatg gccaatccgt ttttcacttc
      481 tgctgaggtg atcaaacagc tcaaagcatc ccaagctaag ctcataatta cgcaagcttg
      541 ttacgtagac aaagtgaaag actacgcagc agagaaaaat atacagatca tttgcatcga
      601 tgatgctcct caggattgtt tacatttctc caaacttatg gaagctgatg aatcagaaat
      661 gcctgaggtt gtgatcaatt cagacgatgt cgtcgcgtta ccttactcat cgggtactac
      721 aggactaccg aaaggtgtta tgttgacaca caaaggactt gttactagcg tggcacaaca
      781 agttgatgga gacaatccga atttatatat gcatagcgag gatgtgatga tctgcatatt
      841 gcctt
//
