LOCUS       sequence-139235-        9536 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the PlexinD1 protein, 
            C-terminally fused to the Fc region of human IgG1 + 6X histidine 
            tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9536)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9536)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnd1-Fc-His
FEATURES             Location/Qualifiers
     source          1..9536
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6116..6133
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6159..6280
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6196..6215)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6250..6269
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6318..6335)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6489..7077)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6569..6588)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7248..8039,8040..8108))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7871..7890
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8109..8213)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8281..8299
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8337..8359)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8361..8381)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8366..8696
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8547..8682
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8609..8628
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8833..8852
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8919..9374)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9056..9077)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9268..9287
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9500..9522
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9514..9531
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9515..9531
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggctcgtcg
     1621 cgccgcgggc ggcgcacccc ctagcgcccg ggccgccgcg gccgtcccct tgcgtccgcg
     1681 ccctcactcg cggggccctg gtctgctgcc gctgcctctg ctgctgctgc tcggggcggc
     1741 acgggccggc gccctagaga tccagcgccg tttcccctcg cccacgccca ccaacaactt
     1801 cgccctggac ggcacggcgg gcaccgtgta cttggcggca gtgaaccgcc tgtaccaact
     1861 gtcgagtgcc aacttgagcc tggaagccga ggcgaccgtg ggtcccgtgc cggacagccc
     1921 gctgtgtcac gccccgcagc tcccgcaggc ctcgtgcgag cacccgcggc gcctcacgga
     1981 caactacaac aaaatcctgc agttggaccc gggccagggt ctggtggtcg cgtgcggctc
     2041 catctaccag ggtctgtgcc agctgaggcg ccggggcaac atctcagccc tggccgtgag
     2101 ctttccgcct gccgcgccga ccgcagaacc ggtcaccgtg ttccccagca tgctcaacgt
     2161 ggccgccaac caccccaacg cgtccactgt gggactggtg ctgccgccta cctcgggcac
     2221 cgggggcagc cgtctgctcg tgggcgccac gtacaccggc ttcggcagcg ctttcttccc
     2281 gcgcaaccgt agcctagaag accaccgctt cgagaacacg cccgagatcg ctatccgctc
     2341 cctggacgcg cgtggagact tggccaagct cttcaccttc gaccttaacc cgtcggacga
     2401 taacatcctg aagatcaagc agggcgccaa ggagcagcac aagctgggct tcgtgcgtgc
     2461 cttcttgcac ccggcggtgc caccgcacag cgcgcagccc tacgcgtacc tggcgctcaa
     2521 cagcgaggcg cgtgcgggcg acaaggacag ccaggcgcgc agcctgctgg cgcgcatctg
     2581 cctgccccgc ggcgcgggtg gcgacgccaa gaagctcacc gagtcctaca tccaactggg
     2641 cttgcagtgc gcgggcggcg cgggccgcgg cgacctctac agccgcctcg tgtcggtttt
     2701 ccccgcgcgc gagcagttct tcgctgtctt cgagaggccc cagggcgccc caggtgcccg
     2761 caacgccccg gccgcgcttt gcgccttccg cttcgacgac gtgcaggctg ccattcgtgc
     2821 agcgcgcacc gcctgcttcg tggagccggc gcccgacgtg gtggcggtgt tggacagtgt
     2881 ggtgcagggc accgggccgg cctgcgagag caagcgcaac atacagctgc agccggagca
     2941 actggattgc ggagcggccc acctgcagca cccactgacc atcctgcagc cgctgagggc
     3001 atcgcccgtg ttccgtgctc cagggctcac ggccgtggct gtggccagtg ccaacaacta
     3061 cacggccgtc tttctgggca ccgccacagg gaggctcctc aagatcagcc tgaacgagag
     3121 catgcaggta gtaagcaggc gagtgctgac tgtagcctat ggggagcctg tgcatcacgt
     3181 catgcagttt gaccccatgg atcctggtta cctatacctg atgacatccc accagatggc
     3241 ccgagtgaag gtggcagcgt gtgaggtaca ctccacctgc ggggactgcg tgggtgcggc
     3301 cgatgcctac tgtggttggt gcactctgga gacccggtgc acactccagc aggattgcac
     3361 caactccagc cagccacatt tctggaccag tgccagtgag ggccccagcc gctgccctgc
     3421 catgacagta ctgccctcgg agattgatgt gcaccgggac tacacaggta tgatcttaca
     3481 gatctcagga agcctgccca gcctcagcgg catggagatg gcttgtgact atggaaatgg
     3541 cgttcgaacg gtggcccggg tacctggccc tgcctatgat catcagattg cctactgcaa
     3601 tctcctgccc agggcccagt ttccatcctt tcctgctggc caggaccacg tgaccgttga
     3661 gatgtctgta agggtcaaag gacacaacat tgtctcagcc aatttcacca tctacgactg
     3721 cagccgaatt ggacaagtct acccccatac agcctgtacc agctgcctgt ccacacagtg
     3781 gccttgctcc tggtgcatcc agctgcattc atgtgtctcc aaccagtctc agtgccagga
     3841 ctcgccaaac cccacgagtc ctcaggactg tccccagatc ctgccctcgc ccctagcgcc
     3901 cgtgcccaca ggtggctccc aagacatcct ggtgcccctg actaaagcca ccttcttcca
     3961 tggttcctcc ctcgagtgca gctttgggct ggaagagagc tttgaggctg tatgggcgaa
     4021 taactcactg gtccgctgca accaagtggt gctgcacaca acccagaaga gccaggtatt
     4081 tccactgagt ctgaagctga aggggccgcc agaccgattc ctagacagcc ctaaccccat
     4141 gacagttgtg gtctacaact gtgctatggg cagccctgac tgttcccagt gcctgggccg
     4201 tgaggacctg ggtcacctct gtgtttggaa tgatggctgt cgtctaagag ggcccctgca
     4261 gccactccct ggcacctgcc cagcccctga aatccgagcg attgagcctc tgagtggccc
     4321 cttggacggt gggactttgc tgaccatccg tggcaggaac ttgggccgtc ggctcagtga
     4381 tgtggcacat ggtgtgtgga ttggcagtgt ggcctgtgaa cccctggctg acagatacac
     4441 cgtttcagag gagatcgtgt gtgccacagg gcctgccgca ggggccttct cagacgtggt
     4501 aacggtgaac gtctccaaag aaggcaggtc tcgggaacag ttctcctatg tgctgcccac
     4561 agtccactca ctggagcctt ccatgggccc aaaggccggg ggtacaagga tcaccattca
     4621 cggcagtgac ctcaacgtgg gctctatgct ccaggtcctg gtgaatgaca cggacccctg
     4681 cacagatctt acgcgcacag ccaccagcat cacctgcact gtgccagggg gtaccctgcc
     4741 ctctccagtg cctgtgtgtg tgcgcttcga gagccggggc tgcgtgcacg gaaacctcac
     4801 cttctggtac atgcagaacc cagtcatcac agccatcagc ccaggccgca gccctgtcag
     4861 tggcggcagg accatcactg tggctggcga acgcttccac atggtgcaga atgtatcaat
     4921 ggctgtacac cacattggcc gggagcccac gttctgcaag gttctcaact ccacactcat
     4981 cacctgccca tctcctggag ccctgagcaa tgcttcggcg cctgtagact tcttcatcaa
     5041 tggccgggca tatgcagacg aggcagccga ggagctgctg gaccctgcag aggcacagag
     5101 gggcagccgg ttccgcctag actacctccc caacccacag ttctccacag ccaagaggga
     5161 gaagtggatc aaacatcacc caggagagcc gctcaccctc gtcatccata aggagcaaga
     5221 cagcctgggg ctggagagcc atgagtacca catcaagatt ggccaggtgt cctgcgacat
     5281 ccaaatcatc tcagacagag tcatccactg ctcagtcaat gagtcgctgg gcacggctga
     5341 aggacagctg cccatcacaa tccaggtggg gaacttcaac cagaccatcg ccacactgca
     5401 actggggggc agcgagacgg gcgcgcctga caaaactcac acatgcccac cgtgcccagc
     5461 acctgaactc ctggggggac cgtcagtctt cctcttcccc ccaaaaccca aggacaccct
     5521 catgatctcc cggacccctg aggtcacatg cgtggtggtg gacgtgagcc acgaagaccc
     5581 tgaggtcaag ttcaactggt acgtggacgg cgtggaggtg cataatgcca agacaaagcc
     5641 gcgggaggag cagtacaaca gcacgtaccg tgtggtcagc gtcctcaccg tcctgcacca
     5701 ggactggctg aatggcaagg agtacaagtg caaggtctcc aacaaagccc tcccagcccc
     5761 catcgagaaa accatctcca aagccaaagg gcagccccga gaaccacagg tgtataccct
     5821 gcccccatcc cgggatgagc tgaccaagaa ccaggtcagc ctgacctgcc tggtcaaagg
     5881 cttctatccc agcgacatcg ccgtggagtg ggagagcaat gggcagccgg agaacaacta
     5941 caagaccacg cctcccgtgc tggactccga cggctccttc ttcctctaca gcaagctcac
     6001 cgtggacaag agcaggtggc agcaggggaa cgtcttctca tgctccgtga tgcatgaggc
     6061 tctgcacaac cactacacgc agaagagcct ctccctgtct ccgggtaaag gcggccatca
     6121 tcaccatcac cattaatcta gagatcttat taaagcagaa cttgtttatt gcagcttata
     6181 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     6241 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg tcgactctag
     6301 ctagactctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     6361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     6421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     6481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     6541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     6601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     6661 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     6721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     6781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     6841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     6901 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     6961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     7021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     7081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     7141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     7201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     7261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     7321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     7381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     7441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     7501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     7561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     7621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     7681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     7741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     7801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     7861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     7921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     7981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     8041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     8101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     8161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     8221 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     8281 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     8341 tgacacatgc agctcccgga ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     8401 cccaggcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa
     8461 agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa
     8521 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8581 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct
     8641 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     8701 tcccgggagc ttgtatatcc attttcgtcc cggagacggt cacagcttgt ctgtaagcgg
     8761 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct
     8821 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     8881 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaagcg ttaatatttt
     8941 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat
     9001 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt
     9061 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt
     9121 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag
     9181 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg
     9241 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc
     9301 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc
     9361 gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
     9421 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
     9481 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgaatt
//
