LOCUS       sequence-139256-        9191 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the N-terminal extracellular region of the PlexinB2 
            protein following proteolytic cleavage (ie, short), C-terminally 
            fused to the Fc region of human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9191)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9191)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb2(S)-Fc-His
FEATURES             Location/Qualifiers
     source          1..9191
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3086..3097
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             5771..5788
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    5814..5935
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5851..5870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5905..5924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5973..5990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6144..6732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6224..6243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6903..7694,7695..7763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7526..7545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7764..7868)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7936..7954
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(7992..8014)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8016..8036)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8021..8351
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8202..8337
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8488..8507
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(8574..9029)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8711..8732)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8923..8942
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9155..9177
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9169..9186
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9170..9186
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggctctgcc
     1621 actctgggcc ctgacctttc taggtctcac gggcctaggg ttgagcctgc ggtcccgaaa
     1681 gccggagagt ttccgcagtg agacagagct gaaccacctg gctgtggatg aggtcacagg
     1741 tgtggtgtat gtcggggcag tgaacgcgct ctaccagctg agtgccgacc tgcacgtcca
     1801 gcagcacgtg gtcacaggcc ccttcatgga taacaagaag tgcacaccac ccattgaggc
     1861 cagccagtgc cacgaagcgg tgctcactga caacttcaac cagctgctgc tgctcgaccc
     1921 accggggaaa cgcctggtgg agtgcggcag cctcttcaag ggcatctgtg ccctgcgcgc
     1981 catgagcaac atctcggtgc gcctcttcta cgaggatggc agcggcgaga agtccttcgt
     2041 ggccagcaat gacgagagag tggccaccgt ggggctggtg acctccacgc gccccgacgg
     2101 cgagcgcgtg ctgtttgtgg gcaaaggcaa tgggccgcac gacaatggta tcatcgtgag
     2161 cacccgcctg ctggacaggg ctgagggccg ggaggccttt gaggcctact ctgaccacac
     2221 caccttcaag gccggctacc tgtccacgaa tactcagcag tttgtcgcag cctttgagga
     2281 tgatttctat gtcttcttcg tctttaacca tcaagacaag catcctgcta agaaccgcac
     2341 gctgctggcg cgcatgtgca aagacgaccc ctcctactat tcctatgtag agatggacct
     2401 gcagtgtcag gatcccagtg acccccaaga ctctgccttt ggcacctgcc tagccgcttc
     2461 tgtagccaca tcgggtgctg gcagagcact ttatgctgtc ttcagcagag atggccggag
     2521 cactggggga cctggcgcgg gcctctgtgt gttcccgcta gataaagtcc gtgagaagat
     2581 agaagccaac cgtaacgcct gctacacagg tgcccgagag gcaggccgca ctatcttcta
     2641 caagcccttc cacggagaaa tccagtgtgg cggccatctg ataggtgcca gcgagagctt
     2701 cccatgcggc tcggagcatc tgccctatcc actgggcagt cgtgatggac tcgtcgccac
     2761 agccgtgctg caccgcgggg gcctgaatct gacagcagtg acagtaactg ccgagaatga
     2821 ccacactgtt gcgttcctgg gcacctcaga tggccggatc cttaaggtgt accttgctcc
     2881 agacggcact tctgcagagt atggttctat cccagtagac atcaataaga aaatcaagca
     2941 ggacctggca ctgtctggaa acctgtccag tctgtatgct atgacccagg acaaggtgtt
     3001 ccggctccca gtacaagaat gcctgagcta tgtaacctgc gctcagtgtc gtgactccca
     3061 agatccctac tgtggctggt gtgtcatcga gggacgatgc accaggaagt ccgagtgctc
     3121 acgggccgag gaaaccggac actggctgtg gagccgggag aagtcctgtg tggccatcac
     3181 tgatgccttt ccacagaaca tgagccggcg ggcccaagga gaggtacgcc tgtctgtcag
     3241 ccccctgccc accctgactg aggacgatga gttactgtgc ctctttggtg actcaccacc
     3301 ccaccctgcc cgggtagagg acgataccgt catctgtaat tccccaagca gcatccctag
     3361 tacaccgcca ggccaagacc atgttgacgt gagcatccag ctcctcttaa aaagcggcag
     3421 tgtcttcctc acctcccacc agtatccctt ctatgactgc cgtgaggcca tgagcctggt
     3481 ggagaacctg ccgtgcatct cttgtgctag caaccgctgg acttgccaat gggacctgca
     3541 gtactacgag tgtcgggagg cttcgcccaa cccagaggag ggaatcatac gtgcccacat
     3601 ggaggacaac tgcccccagt tcctggcccc tgaccctttg gtcatcccca tgaaccatga
     3661 gacagaggtg accttccagg gcaagaactt ggagactgtg aaggtttctt ccctgtatgt
     3721 gggcagtgag ctgctgaatt ttgaagagac tgtgaccatg catgaatcag acaccttctc
     3781 ttttaggacc ccaaagctat cccatgatgg taatgagaca ctgcctcttc acctgtatgt
     3841 taagtccttt ggcaagaaca ttgacagcaa gctacaagtg actctctaca actgctcctt
     3901 tggccgcagt gactgtagcc tgtgtctggc tgccgatcct gcctacaggt gtgtgtggtg
     3961 ccgtgggcag aaccggtgcg tgtacgaagc tctgtgcagc aatgtcactt ctgagtgccc
     4021 accaccagtt atcactagga tccagcctga gacgggcccg ctgggtgggg gcatcctggt
     4081 cactatccat gggtccaatc tgggtgtcaa agcagatgac gtcaagaaga taactgtggc
     4141 tggccagaac tgtgcctttg aaccaagagg gtactccgta tccacccgga ttgtgtgtgc
     4201 aattgaggct tcggagatgc ccttcacagg aggcattgag gtggatgtta atggaaaact
     4261 cggccattca ccgccacacg tccagttcac ttatcaacaa ccccagcctc tcagtgtgga
     4321 gccacgacag gggccacagg caggtggcac cacattgacc atcaatggca ctcacctgga
     4381 cacaggctcc aaggaggatg tgcgggtgac actcaatgac gtcccttgtg aagtgacaaa
     4441 gtttggagca cagctacagt gtgtcacagg tcaacagttg gctccaggcc aggtgacact
     4501 agaaatctac tatgggggct ccagagtgcc cagccccggc atctctttca cctactgcga
     4561 gaaccccatg atacgagcct ttgagccatt gagaagcttt gtcagtggtg gccggagcat
     4621 caacgttact ggccagggct tcagcctcat ccagaagttt gccatggttg tcatcgctga
     4681 gcccttgcgg tcctggaggc ggcggcggcg ggaggctgga gccctggagc gtgtgacggt
     4741 cgagggcatg gagtacgtgt tctacaacga caccaaggtc gtcttcttgt ctcctgctgt
     4801 ccccgaagag cccgaggctt acaacctcac cgtgctgata cggatggacg gtcactgtgc
     4861 cccgctcagg accgaagccg gagtctttga gtatgtggca gaccccacct ttgagaactt
     4921 cacgggtggc gtcaaaaagc aggtcaacaa gctcatccac gcccggggaa ccaatctgaa
     4981 caaagccatg actctggagg aggcagaggc atttgtgggt gctgagcgtt gcattatgaa
     5041 gacgctgacc gagacggacc tgtactgcga gcccggcgcg cctgacaaaa ctcacacatg
     5101 cccaccgtgc ccagcacctg aactcctggg gggaccgtca gtcttcctct tccccccaaa
     5161 acccaaggac accctcatga tctcccggac ccctgaggtc acatgcgtgg tggtggacgt
     5221 gagccacgaa gaccctgagg tcaagttcaa ctggtacgtg gacggcgtgg aggtgcataa
     5281 tgccaagaca aagccgcggg aggagcagta caacagcacg taccgtgtgg tcagcgtcct
     5341 caccgtcctg caccaggact ggctgaatgg caaggagtac aagtgcaagg tctccaacaa
     5401 agccctccca gcccccatcg agaaaaccat ctccaaagcc aaagggcagc cccgagaacc
     5461 acaggtgtat accctgcccc catcccggga tgagctgacc aagaaccagg tcagcctgac
     5521 ctgcctggtc aaaggcttct atcccagcga catcgccgtg gagtgggaga gcaatgggca
     5581 gccggagaac aactacaaga ccacgcctcc cgtgctggac tccgacggct ccttcttcct
     5641 ctacagcaag ctcaccgtgg acaagagcag gtggcagcag gggaacgtct tctcatgctc
     5701 cgtgatgcat gaggctctgc acaaccacta cacgcagaag agcctctccc tgtctccggg
     5761 taaaggcggc catcatcacc atcaccatta atctagagat cttattaaag cagaacttgt
     5821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5941 tctggtcgac tctagctaga ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     6001 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     6061 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     6121 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     6181 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     6241 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     6301 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     6361 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     6421 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     6481 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     6541 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     6601 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     6661 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     6721 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     6781 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     6841 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     6901 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     6961 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     7021 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     7081 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     7141 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     7201 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     7261 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     7321 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     7381 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     7441 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     7501 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     7561 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     7621 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     7681 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     7741 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     7801 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     7861 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     7921 acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat
     7981 gacggtgaaa acctctgaca catgcagctc ccggaggaat gtgtgtcagt tagggtgtgg
     8041 aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     8101 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     8161 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8221 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8281 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8341 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cgtcccggag acggtcacag
     8401 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
     8461 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     8521 atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggaaattgt
     8581 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
     8641 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
     8701 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
     8761 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
     8821 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
     8881 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
     8941 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
     9001 cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg
     9061 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     9121 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     9181 gccagtgaat t
//
