LOCUS       sequence-139321-         608 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Deoxyviolacein pathway (vioABEC) in monocistronic configuration, 
            transcriptionally driven by orthogonal T7-lac promoter variants G6 
            (vioABE) and 4A6 (vioC) for orthogonal flux redirection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Cress BF, Jones JA, Kim DC, Leitz QD, Englaender JA, Collins SM, 
            Linhardt RJ, Koffas MA
  TITLE     Rapid generation of CRISPR/dCas9-regulated, orthogonally repressible
            hybrid T7-lac promoters for modular, tuneable control of metabolic 
            pathway fluxes in Escherichia coli.
  JOURNAL   Nucleic Acids Res. 2016 Apr 13. pii: gkw231.
  PUBMED    27079979
REFERENCE   2  (bases 1 to 608)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 13. pii: gkw231."
COMMENT     Sequence Label: pETM6-G6-vioABE-4A6-vioC
FEATURES             Location/Qualifiers
     source          1..608
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    57..81
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             96..118
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 tagcctagga tcgagatcga tctcgatccc gcgaaattaa tacgactcac tatttcggaa
       61 ttgtgagcgg ataacaattc ccctctagaa ataattttgt ttaactttaa gaaggagata
      121 tacatatgtt aaatttagag cattcagttt caattgtcgg agctggaatt tctgggatag
      181 tgtgtgcaat tacattagct cgttcaaggg cattagaagg ttacgcaata gatgtctatg
      241 aacaaagccc ccgcgttggt ggacggatcc aaacaaactt gttgagcaat cattacatcg
      301 atttaggtgc cgctcgtttt tgttcacaat tgcatggtaa tgtatatgat ctagtcaaga
      361 ggtttgaact cgaatttgaa ccgtttcctt ttactcaact tagatttact cagccaattc
      421 aaaaagaact caagactatt ttgtctaatc taaaaccgtt aatcgatgaa cataaagaag
      481 attcttttgt cgaatttgtt acgagctatt tgggtaaaga aaaagcaaaa gcagtaatct
      541 tagcactggg ctacgactca ctctctatgc caataatttc tccacatatt gcttacgata
      601 tcattgaa
//
