LOCUS       sequence-139403-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for C-terminal Twin-Strep tag, Puromycin-resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pSK24
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             14..37
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             74..97
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    complement(107..140)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gctcgagagc gcttggagcc acccgcagtt cgagaaaggt ggaggttccg gaggtggatc
       61 gggaggtgga tcgtggagcc acccgcagtt cgaaaaatga actagtgaag ttcctatact
      121 ttctagagaa taggaacttc aaataaacat atgggcgcgc cctgttggaa tatactattc
      181 aacctacaaa aataacgtta aacaacacta ctttatattt gatatgaatg gccacacctt
      241 ttatgccata aaacatattg taagagaata ccactctttt tattccttct ttccttcttg
      301 tacgtttttt gctgtgagta ggtcgtggtg ctggtgttgc agttgaaata acttaaaata
      361 taaatcataa aactcaaaca taaacttgac tatttattta tttattaaga aaggaggtac
      421 cggtggcgcg ccatgaccga gtacaagcct acagtgcgac tggccacacg cgatgacgtt
      481 cccagagccg ttcggacgtt ggctgccgcg tttgcggact atcccgcaac tcgccatact
      541 gtggaccctg atcgccacat cgagagggtg actgagcttc aggagctgtt cctgaccagg
      601 gtagggctgg acataggcaa ggtctgggtg gccgacgatg gagccgctgt tgcggtgtgg
      661 actaccccag agagcgtgga agcaggggct gtctttgccg aaattgggcc aaggatggcc
      721 gaactgtctg ggtcccgttt ggctgcccaa cagcagatgg aaggactgct tgccccacat
      781 cgacccaagg agcctgcatg gttcctcgct accgtgggtg tgagcccaga tcaccagggc
      841 aaaggtctgg gatcagccgt cgttctgcct ggcgtcgagg ctgctgagag agcaggagta
      901 c
//
