LOCUS       sequence-139415-         922 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Thermoplasma acidophilum citrate synthase with C-terminal 
            His6 tag (TpCSH6).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Murphy JR, Donini S, Kappock TJ
  TITLE     An active site-tail interaction in the structure of 
            hexahistidine-tagged Thermoplasma acidophilum citrate synthase.
  JOURNAL   Acta Crystallogr F Struct Biol Commun. 2015 Oct 1;71(Pt 10):1292-9. 
            doi: 10.1107/S2053230X15015939. Epub 2015 Sep 23.
  PUBMED    26457521
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Acta 
            Crystallogr F Struct Biol Commun."; date: "2015-10-1"; volume: 
            "71(Pt 10):1292-9. doi"; pages: " 10.1107/S2053230X15015939. Epub 
            2015 Sep 23"
COMMENT     Sequence Label: pJK438
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatgccagaa actgaagaaa ttagcaaagg
       61 gcttgaggat gtcaatataa agtggacaag gctcacgacc atcgacggaa ataagggaat
      121 cctgcgatac gggggctatt ctgtcgaaga tataattgcc tctggggcac aggatgagga
      181 gatccagtat ctgttcctgt atgggaacct tccaactgaa caggagctca gaaaatacaa
      241 ggaaaccgtc cagaagggtt ataagatacc agattttgtc ataaacgcca taaggcagct
      301 gccaagggaa tcggatgctg tggcaatgca gatggccgcg gttgcagcca tggcagcatc
      361 ggagacaaag ttcaagtgga acaaggatac ggacagagac gttgcagcag aaatgatcgg
      421 caggatgtca gcgataaccg tgaacgtgta caggcacata atgaacatgc cggccgagct
      481 tccgaaacca tcagacagct acgctgagag cttcctgaac gctgcgtttg gaaggaaggc
      541 aacaaaggaa gagatagacg ccatgaatac ggcactgatt ctctacacag accatgaggt
      601 gcctgcatct accacggcag gcttggtcgc ggtatcaacc ctctcagaca tgtactccgg
      661 cataactgca gctcttgcgg ccctcaaggg tccgctgcat ggcggtgcag ctgaggcagc
      721 aatagcgcag ttcgatgaga taaaggatcc cgccatggtg gagaagtggt tcaacgacaa
      781 cataataaac ggaaagaaga gactcatggg cttcggccac agggtataca agacctacga
      841 tccaagggca aagatattca agggcatcgc tgagaaactc tccagcaaga agccagaggt
      901 gcacaaggta tatgagatcg cc
//
