LOCUS       sequence-139429-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mutant human PUM1-HD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Cheong CG, Hall TM
  TITLE     Engineering RNA sequence specificity of Pumilio repeats.
  JOURNAL   Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13635-9. Epub 2006 Sep
            5.
  PUBMED    16954190
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2006-09-12"; volume: "103(37)"; pages: 
            "13635-9. Epub 2006 Sep 5"
COMMENT     Sequence Label: pTYB3-human PUM1-HD MUT6-2 7-2
            (N1043S-Q1047E-...
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataac catgggccgc agccgccttt tggaagattt
       61 tcgaaacaac cggtacccca atttacaact gcgggagatt gctggacata taatggaatt
      121 ttcccaagac cagcatgggt ccagattcat tcagctgaaa ctggagcgtg ccacaccagc
      181 tgagcgccag cttgtcttca atgaaatcct ccaggctgcc taccaactca tggtggatgt
      241 gtttggtaat tacgtcattc agaagttctt tgaatttggc agtcttgaac agaagctggc
      301 tttggcagaa cggattcgag gccacgtcct gtcattggca ctacagatgt atggctgccg
      361 tgttatccag aaagctcttg agtttattcc ttcagaccag cagaatgaga tggttcggga
      421 actagatggc catgtcttga agtgtgtgaa agatcagaat ggcaatcacg tggttcagaa
      481 atgcattgaa tgtgtacagc cccagtcttt gcaatttatc atcgatgcgt ttaagggaca
      541 ggtatttgcc ttatccacac atccttatgg ctgccgagtg attcagagaa tcctggagca
      601 ctgtctccct gaccagacac tccctatttt agaggagctt caccagcaca cagagcagct
      661 ggtacaggat caatatggaa gttatgtaat cgaacatgta ctggagcacg gtcgtcctga
      721 ggataaaagc aaaattgtag ca
//
