LOCUS       sequence-139459-        9006 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mammalian expression of DHX36.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9006)
  AUTHORS   Sexton AN, Collins K
  TITLE     The 5' guanosine tracts of human telomerase RNA are recognized by 
            the G-quadruplex binding domain of the RNA helicase DHX36 and 
            function to increase RNA accumulation.
  JOURNAL   Mol Cell Biol. 2011 Feb;31(4):736-43. doi: 10.1128/MCB.01033-10. 
            Epub 2010 Dec 13.
  PUBMED    21149580
REFERENCE   2  (bases 1 to 9006)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01033-10"; journalName: "Mol Cell Biol"; date: 
            "2011-02"; volume: "31"; issue: "4"; pages: "736-43"
COMMENT     Sequence Label: pcDNA3.1 TAP DHX36
FEATURES             Location/Qualifiers
     source          1..9006
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        381..584
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     535..555
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     629..648
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        629..647
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             712..885
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             886..1059
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             1117..1137
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             1147..1224
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
     primer_bind     complement(4363..4380)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4369..4593
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4639..5067
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4726..4745)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4936..4957
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5076..5096)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5081..5410
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5261..5396
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5323..5342
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5477..6271
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5531..5550)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6141..6160
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6445..6566
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6482..6501)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6536..6555
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6615..6631)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6615..6631)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6628..6650)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6639..6655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6663..6669,6670..6687,6688..6693))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6708..6729
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6846..6863)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7017..7605)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7097..7116)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7776..8567,8568..8636))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8399..8418
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8637..8741)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8816..8835)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
       61 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
      121 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
      181 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
      241 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
      301 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
      361 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
      421 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
      481 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
      541 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag
      601 aacccactgc ttactggctt atcgaaatta atacgactca ctatagggag acccaagctg
      661 gctagagctg ccaccatgaa aaccgcggct cttgcgcaac acgatgaagc cgtggacaac
      721 aaattcaaca aagaacaaca aaacgcgttc tatgagatct tacatttacc taacttaaac
      781 gaagaacaac gaaacgcctt catccaaagt ttaaaagatg acccaagcca aagcgctaac
      841 cttttagcag aagctaaaaa gctaaatgat gctcaggcgc cgaaagtaga caacaaattc
      901 aacaaagaac aacaaaacgc gttctatgag atcttacatt tacctaactt aaacgaagaa
      961 caacgaaacg ccttcatcca aagtttaaaa gatgacccaa gccaaagcgc taacctttta
     1021 gcagaagcta aaaagctaaa tggtgctcag gcgccgaaag tagacgcgaa ttccgcgggg
     1081 aagtcaaccg attatgatat tccaactact gctagcgaga atttgtattt tcagggttcc
     1141 atggaaaaga gaagatggaa aaagaatttc atagccgtct cagcagccaa ccgctttaag
     1201 aaaatctcat cctccggggc acttggagca ggtgctaagc ttatgagtta tgactaccat
     1261 cagaactggg gccgtgatgg gggtccccgc agctccggtg ggggctatgg aggggggcca
     1321 gcagggggtc atggaggtaa ccgaggctcc ggaggaggcg gcggcggcgg agggggtggt
     1381 cgaggcggca ggggccggca tcccgggcac ctgaaaggcc gcgaaatcgg catgtggtac
     1441 gcgaaaaaac aggggcagaa gaacaaggaa gcggagaggc aagagagagc tgtagtacac
     1501 atggatgaac gacgagaaga acaaattgta cagttactga attctgttca agcgaagaat
     1561 gataaagagt cagaagcaca gatatcctgg tttgctcctg aggatcatgg atacggtact
     1621 gaagtttcta ctaagaacac accatgctca gagaacaaac ttgacatcca ggaaaagaag
     1681 ttgataaatc aagaaaaaaa aatgtttaga atcaggaaca gatcatatat tgaccgagat
     1741 tctgagtatc tcttgcaaga aaatgaacca gatggaactt tagaccaaaa attattggaa
     1801 gatttacaaa agaaaaaaaa tgaccttcgg tatattgaaa tgcagcattt cagagaaaag
     1861 ctgccttcgt atggaatgca aaaggaattg gtaaatttaa ttgataacca tcaggtaaca
     1921 gtaataagcg gtgaaactgg ttgtggcaaa accactcaag ttactcagtt cattttggat
     1981 aactacattg aaagaggaaa aggatctgct tgcagaatag tttgtactca gccaagaaga
     2041 attagtgcca tttcagttgc ggaaagagta gctgcagaaa gggcagaatc ttgtggcagt
     2101 ggtaatagta ctggatatca aattcgtctc cagagtcggt tgccaaggaa acagggttct
     2161 atcttatact gtacaacagg aatcatcctt cagtggctcc agtcagaccc gtatttgtcc
     2221 agtgttagtc atatcgtact tgatgaaatc catgaaagaa atctgcagtc agatgtttta
     2281 atgactgttg ttaaagacct tctcaatttt cgatctgact tgaaagtaat attgatgagt
     2341 gcaacattga atgcagaaaa gttttcagaa tattttggta actgtccaat gatacatata
     2401 cctggtttta cctttccggt tgtggaatat cttttggaag atgtaattga aaaaataagg
     2461 tatgttccag aacaaaaaga acacagatgc cagtttaaga ggggtttcat gcaagggcat
     2521 gtaaatagac aagaaaaaga agaaaaagaa gcaatatata aagaacgttg gccagattat
     2581 gtaagggaac tgcgaagaag gtattctgca agtactgtag atgttataga aatgatggag
     2641 gatgataaag ttgatctgaa tttgattgtt gccctcatcc gatacattgt tttggaagaa
     2701 gaggatggtg cgatactggt ctttctgcca ggctgggaca atatcagcac tttacatgat
     2761 ctcttgatgt cacaagtaat gtttaaatca gataaatttt taattatacc tttacattca
     2821 ctgatgccta cagttaacca gacacaggtg tttaaaagaa cccctcctgg tgttcggaaa
     2881 atagtaattg ctaccaacat tgcggagact agcattacca tagatgatgt cgtttatgtg
     2941 atagatggag gaaaaataaa agagacgcat tttgatactc agaacaatat cagtacaatg
     3001 tccgctgagt gggttagtaa agctaatgcc aaacagagaa aaggtcgagc tggaagagtt
     3061 caacctggtc attgctatca tctgtataat ggtcttagag caagtcttct agatgactat
     3121 caactgccag aaattttgag aactcctttg gaagaacttt gtttacaaat aaagatttta
     3181 aggctaggtg gaattgctta ttttctgagt agattaatgg acccaccatc aaatgaggca
     3241 gtgttactct ccataagaca cctgatggag ctgaacgctt tggataaaca agaagaattg
     3301 acacctcttg gagtccactt ggcacgatta cccgttgagc cacatattgg aaaaatgatt
     3361 ctttttggag cactgttctg ctgcttagac ccagtactca ctattgctgc tagtctcagt
     3421 ttcaaagatc catttgtcat tccactggga aaagaaaaga ttgcagatgc aagaagaaag
     3481 gaattggcaa aggatactag aagtgatcac ttaacagttg tgaatgcgtt tgagggctgg
     3541 gaagaggcta ggcgacgtgg tttcagatac gaaaaggact attgctggga atattttctg
     3601 tcttcaaaca cactgcagat gctgcataac atgaaaggac agtttgctga gcatcttctt
     3661 ggagctggat ttgtaagcag tagaaatcct aaagatccag aatctaatat aaattcagat
     3721 aatgagaaga taattaaagc tgtcatctgt gctggtttat atcccaaagt tgctaaaatt
     3781 cgactaaatt tgggtaaaaa aagaaaaatg gtaaaagttt acacaaaaac cgatggcctg
     3841 gttgctgttc atcctaaatc tgttaatgtg gagcaaacag actttcacta caactggctt
     3901 atctatcacc taaagatgag aacaagcagt atatacttgt atgactgcac agaggtttcc
     3961 ccatactgtc tcttgttttt tggaggtgac atttccatcc agaaggataa cgatcaggaa
     4021 actattgctg tagatgagtg gattgtattt cagtctccag caagaattgc ccatcttgtt
     4081 aaggaattaa gaaaggaact agatattctt ctgcaagaga agattgaaag tcctcatcct
     4141 gtagactgga atgacactaa atccagagac tgtgcagtac tgtcagctat tatagacttg
     4201 atcaaaacac aggaaaaggc aactcccagg aactttccgc cacgattcca ggatggatat
     4261 tacagctgag gatccactag tccagtgtgg tggaattctg cagatatcca gcacagtggc
     4321 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgact gtgccttcta
     4381 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca
     4441 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc
     4501 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata
     4561 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg
     4621 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4681 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4741 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4801 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     4861 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4921 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
     4981 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     5041 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg
     5101 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5161 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5221 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5281 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5341 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5401 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc aagagacagg
     5461 atgaggatcg tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg
     5521 ggtggagagg ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc
     5581 cgtgttccgg ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg
     5641 tgccctgaat gaactgcagg acgaggcagc gcggctatcg tggctggcca cgacgggcgt
     5701 tccttgcgca gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg
     5761 cgaagtgccg gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat
     5821 catggctgat gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca
     5881 ccaagcgaaa catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca
     5941 ggatgatctg gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa
     6001 ggcgcgcatg cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa
     6061 tatcatggtg gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc
     6121 ggaccgctat caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga
     6181 atgggctgac cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc
     6241 cttctatcgc cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac
     6301 caagcgacgc ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg
     6361 ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc
     6421 atgctggagt tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa
     6481 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
     6541 ttgtccaaac tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc
     6601 ttggcgtaat catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca
     6661 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
     6721 ctcacattaa ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag
     6781 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     6841 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     6901 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     6961 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     7021 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     7081 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     7141 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     7201 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     7261 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     7321 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     7381 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     7441 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     7501 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     7561 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     7621 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     7681 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     7741 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     7801 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     7861 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     7921 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     7981 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     8041 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     8101 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     8161 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     8221 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     8281 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     8341 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     8401 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     8461 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     8521 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     8581 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     8641 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     8701 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     8761 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     8821 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     8881 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     8941 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata
     9001 cgcgtt
//
