LOCUS       sequence-139555-         913 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 2xNLS-mCherry + Cbr-unc-119 donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 913)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 913)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS292
FEATURES             Location/Qualifiers
     source          1..913
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    9..108
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(397..430)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(579..590)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 ctggatggca aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg
       61 ataagcaatg ctttcttata atgccaactt tgtacaagaa agctgggtgc tcttcgacct
      121 taattttcaa cttccttggc aagcttcttc gcgttttcac tcagttttgt cggattcgct
      181 tttcgtctac ggctcataga gctcccctta tacaattcat ccatgccacc tgtcgagtgc
      241 cgcccttcag ctctttcata ttgctcaacg atagtgtaat cctcgttgtg ggaggtgata
      301 tccagtttaa tattaacatt gtacgctcct ggcagctgaa ctggtttttt ggctttgtaa
      361 gttgtcttca cctgaaaatt taaatcagat tagttaataa cttcgtatag catacattat
      421 acgaagttat ctttgagcca atttatccaa gtccttgtaa aaagtattcg aaaattgtta
      481 acggataaaa tgtttattat aatatcaaaa acaatttgtc agttgaccac tttttgatat
      541 atttgacaga aaccgggatg aattggctca aagttagggc gcccttctat tacagggttt
      601 ctgataacaa accggttatt aactcccaac aagggatgat ttcaattcat catgctcaaa
      661 ttgacccaaa ttaagttaca tgacaaattc atcgcccttt tcaactcttc ttggctcatc
      721 atctgttatt ctgttcctat tctgtctgca ccctataccc ctttgcatac tctctcgtca
      781 ttcctttctt ggatagtgtc ttcttctccc cagctctgct acttctatga cttgcgcccg
      841 ctgcttttcc gctcgttcct ctcctctctg acgtcttctt ctgcttcttt tctccttcct
      901 ttttcccttc tca
//
