LOCUS       sequence-139594-        1028 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap flexible linker N-terminal connector donor plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1028)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS288
FEATURES             Location/Qualifiers
     source          1..1028
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     27..43
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     28..44
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(130..146)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(130..146)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(183..203)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(222..238)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(222..238)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(340..359)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(418..437)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 gggcgaattg ggtaccgggc cccccctcga ggtcgacggt atcgataagc ttgatatcga
       61 attcctgcag cccgctcttc gggtggcagc ggaggtaccg gcggtagtgg aggcacgcga
      121 agagcggggg atccactagt tctagagcgg ccgccaccgc ggtggagctc cagcttttgt
      181 tccctttagt gagggttaat tgcgcgcttg gcgtaatcat ggtcatagct gtttcctggc
      241 agctctggcc cgtgtctcaa aatctctgat gttacattgc acaagataaa aatatatcat
      301 catgaacaat aaaactgtct gcttacataa acagtaatac aaggggtgtt atgagccata
      361 ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt
      421 ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga
      481 agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta
      541 cagatgagat ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc
      601 attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag
      661 cattccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag
      721 tgttcctgcg ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg
      781 tatttcgtct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt
      841 ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt
      901 tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt
      961 ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat
     1021 accaggat
//
