LOCUS       sequence-139607-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for N-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS234
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    66..90
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(152..180)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    739..772
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa tgctttttta
       61 taatgccaac tttgtacaaa aaagcaggct cgtaccgaag agcggatcca tgggctcttc
      121 gatgagcaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      181 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      241 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat gggtaagttt
      301 aaacatatat atactaacta accctgatta tttaaatttt cagccaacac ttgtcactac
      361 tttctgttat ggtgttcaat gcttctcgag atacccagat catatgaaac ggcatgactt
      421 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      481 cgggaactac aagacacgta agtttaaaca gttcggtact aactaaccat acatatttaa
      541 attttcaggt gctgaagtca agtttgaagg tgataccctt gttaatagaa tcgagttaaa
      601 aggtattgat tttaaagaag atggaaacat tcttggacac aaattggaat acaactataa
      661 ctcacacaat gtatacatca tggcagacaa acaaaagaat ggaatcaaag ttgtaagttt
      721 aaacatgatt ttactaacat aacttcgtat aatgtatgct atacgaagtt atttctagac
      781 attctctaat gaaaaaatct ttcagttgaa attgaaaatg agttaaagtt ggagttttta
      841 ttgaaaacag atttccgtgt gattagtgtt tttagcgagt gtgacaggac agcgaaaaaa
      901 tatagaaaca aggggggaac tgaaaagctt aggaatgcat tgaacatgag aaggggaagg
      961 ggaaggaaca aacta
//
