LOCUS       sequence-139610-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for C-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS235
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    65..89
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(184..212)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     protein_bind    771..804
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 tttattttga ctgatagtga cctgttcgtt gcaacaaatt gatgagcaat gcttttttat
       61 aatgccaact ttgtacaaaa aagcaggctc gccgcgaaga gcggatccat gggctcttcg
      121 ggcagcggtg gcagtggagg taccggcgga agcggtagca aaggagaaga acttttcact
      181 ggagttgtcc caattcttgt tgaattagat ggtgatgtta atgggcacaa attttctgtc
      241 agtggagagg gtgaaggtga tgcaacatac ggaaaactta cccttaaatt tatttgcact
      301 actggaaaac tacctgttcc atgggtaagt ttaaacatat atatactaac taaccctgat
      361 tatttaaatt ttcagccaac acttgtcact actttctgtt atggtgttca atgcttctcg
      421 agatacccag atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat
      481 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg taagtttaaa
      541 cagttcggta ctaactaacc atacatattt aaattttcag gtgctgaagt caagtttgaa
      601 ggtgataccc ttgttaatag aatcgagtta aaaggtattg attttaaaga agatggaaac
      661 attcttggac acaaattgga atacaactat aactcacaca atgtatacat catggcagac
      721 aaacaaaaga atggaatcaa agttgtaagt ttaaacatga ttttactaac ataacttcgt
      781 ataatgtatg ctatacgaag ttatttctag acattctcta atgaaaaaat ctttcagttg
      841 aaattgaaaa tgagttaaag ttggagtttt tattgaaaac agatttccgt gtgattagtg
      901 tttttagcga gtgt
//
