LOCUS       sequence-139653-         777 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression plasmid of AsCpf1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 777)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: act-AsCpf1
FEATURES             Location/Qualifiers
     source          1..777
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(121..201)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             complement(208..255)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     regulatory      527..536
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cacttaaaaa ttttatattt accttagagc tttaaatctc tgtaggtagt ttgtccaatt
       61 atgtcacacc acagaagtaa ggttccttca caaagatcct ctagaggtac cctcgagtta
      121 ggcatagtcg gggacatcat atgggtatgc ataatcaggc acgtcgtagg gataagcgta
      181 atctggaaca tcgtatgggt aggatccctt tttctttttt gcctggccgg cctttttcgt
      241 ggccgccggc cttttgttgc gcagctcctg gatgtaggcc agccagtcct gattggagat
      301 gccgttctgc agcttcagat ccttgctctc cttcaggtga ttcagcagca gctggccctt
      361 cagggcgatg tggtaggcgc cattggcatc ggcgtccatg ggccactctg ggttctgaaa
      421 ccgggagtcg aagcacacgc cattcagatc gcgcacgggg ctgttgatat agtcctcgcc
      481 tgtggcggca ttggagttcc gcatctgcag cacgctgcgg atcagggcca ccatggtgtc
      541 gatggcgtga gaatcgtcat tctccagcag ctttggcagg atgttggagc catccctgaa
      601 cacgatgccc ttctcctcca gcagggcgat cagctcgttg gcaggataca ggtcccggta
      661 tctgccggtg aatctgtgat tctcgatcac tggcacgatt ctcttgccgg cgatgaaagg
      721 ggtgcccttg gcgtcaaact gtgtctcgtt cttctcgaac acgatatccc atgcagg
//
