LOCUS       sequence-139742-         661 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of 
            mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 661)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL
FEATURES             Location/Qualifiers
     source          1..661
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      136..145
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             145..174
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttgccacc
      121 ggatcccttg aattcgccac catggagcag aagctgatca gcgaggagga cctgggcctt
      181 tctggcctgc aggccgtcgt tacccaagaa cctagtgtta ccgttagccc aggtggtact
      241 gttatactta cttgtggaag tggtacgggt gccgtcacat ctggtcatta tgcaaattgg
      301 tttcaacaaa aaccaggaca agctccaaga gctttgattt ttgatactga taagaagtat
      361 tcttggaccc caggtagatt ttctggatct ttgctgggag caaaggcagc tttgacaata
      421 tcagatgctc agcctgagga cgaagccgag tattactgtt ctcttagcga cgtggatggc
      481 tacttgtttg gcggtggaac acaactgacg gttctgtccg gtggtggcgg ctctggtggc
      541 ggtggcagcg gcggtggtgg ttccggaggc ggcggttctc aggctgtggt gactcaggag
      601 ccgtcagtga ctgtgtcccc aggagggaca gtcattctca cttgtggctc cggcactgga
      661 g
//
