LOCUS       sequence-139769-         714 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 714)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023."
COMMENT     Sequence Label: pX330-sgRNA_Ago2_2
FEATURES             Location/Qualifiers
     source          1..714
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     114..133
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        213..288
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        385..670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 agataattgg aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta
       61 gaaagtaata atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc
      121 atatgcttac cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
      181 gacgaaacac cggctaagaa ctgttgaatg gagttttaga gctagaaata gcaagttaaa
      241 ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgctt ttttgtttta
      301 gagctagaaa tagcaagtta aaataaggct agtccgtttt tagcgcgtgc gccaattctg
      361 cagacaaatg gctctagagg tacccgttac ataacttacg gtaaatggcc cgcctggctg
      421 accgcccaac gacccccgcc cattgacgtc aatagtaacg ccaataggga ctttccattg
      481 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      541 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattgtgc
      601 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      661 tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct cccc
//
