LOCUS       sequence-139770-         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human codon-optimized SpCas9 and sgRNA targeting Ago2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023."
COMMENT     Sequence Label: pX330-sgRNA_Ago2_2
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..48)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt cgcctcccag
       61 ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc
      121 gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt
      181 gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg
      241 ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc
      301 gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta
      361 gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg agcccttcag
      421 cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca gggccagttc
      481 gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt tttccagctc
      541 gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt tgtagccctt
      601 ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga tggtgatccc
      661 cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt tggccaccac
      721 cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttctta
//
