LOCUS       sequence-139831-         964 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Membrane scaffold protein (MSP), helix 4 deletion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 964)
  AUTHORS   Hagn F, Etzkorn M, Raschle T, Wagner G
  TITLE     Optimized phospholipid bilayer nanodiscs facilitate high-resolution 
            structure determination of membrane proteins.
  JOURNAL   J Am Chem Soc. 2013 Feb 6;135(5):1919-25. doi: 10.1021/ja310901f. 
            Epub 2013 Jan 25.
  PUBMED    23294159
REFERENCE   2  (bases 1 to 964)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ja310901f";
            journalName: "J Am Chem Soc"; date: "2013-02-6- 6"; volume: "135"; 
            issue: "5"; pages: "1919-25"
COMMENT     Sequence Label: pET28a-MSP1D1delltaH4
FEATURES             Location/Qualifiers
     source          1..964
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             66..86
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             625..642
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(695..713)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      709..756
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(880..899)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aataattttg tttaacttta agaaggagat ataccatggg cagcagccat catcatcatc
       61 atcatgaaaa cctgtatttt cagggcagca cctttagcaa actgcgtgaa cagctgggcc
      121 cggtgaccca ggaattttgg gataacctgg aaaaagaaac cgaaggcctg cgtcaggaaa
      181 tgagcaaaga tctggaagag gtgaaagcga aagtgcagcc gctgcgtgcg gaactgcagg
      241 aaggcgcgcg tcagaaactg catgaactgc aggaaaaact gagcccgctg ggcgaagaga
      301 tgcgtgatcg tgcgcgtgcg catgtggatg cgctgcgtac ccatctggcg ccgtatagcg
      361 atgaactgcg tcagcgtctg gcggcccgtc tggaagcgct gaaagaaaac ggcggtgcgc
      421 gtctggcgga atatcatgcg aaagcgaccg aacatctgag caccctgagc gaaaaagcga
      481 aaccggcgct ggaagatctg cgtcagggcc tgctgccggt gctggaaagc tttaaagtga
      541 gctttctgag cgcgctggaa gagtatacca aaaaactgaa cacccagtaa gagctccgtc
      601 gacaagcttg cggccgcact cgagcaccac caccaccacc actgagatcc ggctgctaac
      661 aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc
      721 cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga
      781 ttggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
      841 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
      901 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
      961 ggtt
//
