LOCUS       sequence-139858-        7286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3G, isoform z protein (truncated at cleavage site 
            P1; ie, short), C-terminally fused to the Fc region of human IgG1 + 
            6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3g.z(S)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3866..3883
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    3909..4030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3946..3965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4000..4019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4068..4085)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4239..4827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4319..4338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4998..5789,5790..5858))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5621..5640
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5859..5963)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6031..6049
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6087..6109)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6111..6131)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6116..6446
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6297..6432
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6359..6378
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     6583..6602
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(6669..7124)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6806..6827)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7018..7037
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7250..7272
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7264..7281
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7265..7281
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggacccatc
     1621 tgcgtgggcc atctgctgcc tcctggggag ccttctgttc catgtgggca tccccagccc
     1681 tggtcccagc cccagtgtgc ctcgtctgcg gctctcctac agagatctcc tgtctaccaa
     1741 ccgctctgcc atctttctgg gcccacgggg ttccctagac ctccaagtca tgtacctgga
     1801 tgagtatcga gatcgcctct ttctgggaag ccgagatgcc ctttactctc tgagactgga
     1861 tcaggcatgg ccagaccccc gggaggtctt gtggctgcca cagccaggac agaaggtgga
     1921 atgtgtccgc aagggaaaag accctttgac ggagtgtgcc aacttcgtgc gggtcttaca
     1981 accccataac cggacccatc tgctggcctg tggcacagga gccttccaac ccatctgcac
     2041 cttcatcaca gtggggcatc gaggggagca tgtgctccgc ttggatgcta gcagcgtcga
     2101 gaatggaagg gggcgttgcc cacatgagcc cagccgaccc ttcgccagca cctttgtagg
     2161 tggggagcta tacacaggtc tcactgccga tttcctggga cgtgaggcca tgattttccg
     2221 gagtgggggt ccccgaccag ccctgcgttc tgactctgac cagagcctcc tacatgaacc
     2281 ccggtttgtg atggctgctc ggatcccaga taactctgac cgggacgacg acaaggtgta
     2341 cttcttcttc tccgagactg tcccgtcacc agatggtggc ccaggtcatg tcaccatcag
     2401 ccgagtgggc cgcgtctgtg tgaatgatgc tggtggccag cgggtgctgg tgaacaaatg
     2461 gagcactttc ctcaaggccc ggctggtgtg ctctgtgcct ggcccgggtg gggctgagac
     2521 tcactttgac cagctagagg atgtgttcct gctgtggccc aaggccggga agagcctcga
     2581 ggtgtatgcg ctgttcagca ctgtcagtgc tgtgttccag ggctttgctg tctgtgtgta
     2641 ccacatggta gacatctggg aggtcttcaa tgggcccttt gcccaccgag atggccctca
     2701 gcatcagtgg ggaccctatg ggggcaaggt gcccttccct cgccccggtg tgcctgtgag
     2761 gcctcggcgt ggccgccctg tcctggtcaa aactcacttg gctcagcgac tgcgccagat
     2821 tgtggtggat cgagtggagg ctgaggatgg gacctatgat gtcatcttcc tagggactga
     2881 ttcgggttct gtgctcaaag tcattgccct ccagggtggt ggcttgactg aacctgaaga
     2941 ggtggttttg gaggagctcc aggtgtttaa ggtgccaacg cccatcactg agatggagat
     3001 ctctgtcaaa aggcaaacgc tgtatgtggg ctctccactg ggtgtggccc ggctgcagct
     3061 gcaccagtgt gagacttacg gcagtgcctg tgctgagtgc tgcctggccc gggacccata
     3121 ctgtgcttgg gatggcactg cctgtgcccg ctaccgcccc agctctggcg gcgcgcctga
     3181 caaaactcac acatgcccac cgtgcccagc acctgaactc ctggggggac cgtcagtctt
     3241 cctcttcccc ccaaaaccca aggacaccct catgatctcc cggacccctg aggtcacatg
     3301 cgtggtggtg gacgtgagcc acgaagaccc tgaggtcaag ttcaactggt acgtggacgg
     3361 cgtggaggtg cataatgcca agacaaagcc gcgggaggag cagtacaaca gcacgtaccg
     3421 tgtggtcagc gtcctcaccg tcctgcacca ggactggctg aatggcaagg agtacaagtg
     3481 caaggtctcc aacaaagccc tcccagcccc catcgagaaa accatctcca aagccaaagg
     3541 gcagccccga gaaccacagg tgtataccct gcccccatcc cgggatgagc tgaccaagaa
     3601 ccaggtcagc ctgacctgcc tggtcaaagg cttctatccc agcgacatcg ccgtggagtg
     3661 ggagagcaat gggcagccgg agaacaacta caagaccacg cctcccgtgc tggactccga
     3721 cggctccttc ttcctctaca gcaagctcac cgtggacaag agcaggtggc agcaggggaa
     3781 cgtcttctca tgctccgtga tgcatgaggc tctgcacaac cactacacgc agaagagcct
     3841 ctccctgtct ccgggtaaag gcggccatca tcaccatcac cattaatcta gagatcttat
     3901 taaagcagaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     3961 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4021 atgtatctta tcatgtctgg tcgactctag ctagactctt ccgcttcctc gctcactgac
     4081 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4141 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4201 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4261 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4321 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4381 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4441 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4501 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     4561 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     4621 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     4681 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     4741 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     4801 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     4861 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     4921 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     4981 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     5041 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     5101 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     5161 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     5221 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     5281 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     5341 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     5401 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     5461 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     5521 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     5581 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     5641 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     5701 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     5761 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     5821 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     5881 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     5941 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     6001 accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     6061 gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga ggaatgtgtg
     6121 tcagttaggg tgtggaaagt ccccaggctc cccaggcagg cagaagtatg caaagcatgc
     6181 atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta
     6241 tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc
     6301 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6361 tttatgcaga ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct
     6421 tttttggagg cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcgtcc
     6481 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg
     6541 cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg
     6601 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     6661 gcatcaggaa attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat
     6721 cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata
     6781 gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt
     6841 ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc
     6901 atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa
     6961 agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg
     7021 gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt
     7081 aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtccattc gccattcagg
     7141 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     7201 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     7261 cgttgtaaaa cgacggccag tgaatt
//
