LOCUS       sequence-139864-        7949 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Sema3C protein (truncated at cleavage site P3; ie, 
            long), C-terminally fused to the Fc region of human IgG1 + 6X 
            histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7949)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 7949)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sema3c(L)-Fc-His
FEATURES             Location/Qualifiers
     source          1..7949
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      1604..1613
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4529..4546
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    4572..4693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4609..4628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4663..4682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4731..4748)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4902..5490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4982..5001)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5661..6452,6453..6521))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6284..6303
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6522..6626)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     6694..6712
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(6750..6772)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(6774..6794)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6779..7109
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6960..7095
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7022..7041
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     7246..7265
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(7332..7787)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7469..7490)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7681..7700
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7913..7935
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     7927..7944
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     7928..7944
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tggcattccg
     1621 ggcgatttgt gtgttggttg gagtatttat ttgttccatt tgtgtacgag gatcttccca
     1681 gccccaagca agagtttatt taacatttga tgagcttcga gaaaccaaaa cctctgagta
     1741 ctttagtctg tcccaccagc agttagacta cagaatattg ctgatggatg aagatcaaga
     1801 ccggatatat gtggggagca aagaccacat cctgtctttg aatatcaaca atatcagtca
     1861 agaacctttg agtgttttct ggccagcatc aacaatcaaa gttgaagagt gcaaaatggc
     1921 tggcaaagat cctacacatg gctgtggaaa tttcgtccgg gtgattcaga cattcaaccg
     1981 tactcacctg tatgtctgtg ggagtggagc gttcagccca gtgtgcacct acctgaaccg
     2041 gggaaggagg tcagaggacc aggtattcat gatcgactct aagtgtgaat ctggcaaagg
     2101 acgatgctct ttcaacccga atgtgaacac tgtgtctgtt atgatcaatg aggaactctt
     2161 ctcaggaatg tatatagact tcatgggaac agatgctgct attttccgaa gtttaactaa
     2221 gaggaatgca gttcgaactg atcaacataa ttcaaaatgg ctgagtgaac ccatgtttgt
     2281 ggacgcacat gtgatcccag atggcactga tccaaatgat gctaaggtct atttcttctt
     2341 caaagaaaga ctgactgaca acaataggag cacaaaacag attcattcca tgattgcaag
     2401 aatatgccct aatgacactg gtggacaacg tagtcttgtc aacaagtgga ccacattctt
     2461 aaaggcaaga cttgtatgct cagtcacaga tgaagatggc ccagagacac attttgatga
     2521 actagaggat gtctttctgc tggaaactga caatccaagg acaacactcg tgtatggcat
     2581 cttcaccaca tcaagctctg tttttaaggg atcggcagtg tgtgtgtatc atttatctga
     2641 tatacagact gtattcaatg ggccctttgc ccacaaggaa gggcccaatc accagctgat
     2701 ctcctatcaa ggtagaatcc catatcctcg cccaggaact tgcccaggag gggcctttac
     2761 acccaatatg agaaccacca aggacttccc agatgacgtt gtcactttta ttcggaacca
     2821 ccctctcatg tacaattcca tctaccccat ccacagaagg cctctgatag tccgcatagg
     2881 cactgactac aagtacacaa agattgctgt ggaccgtgtc aacgctgctg atgggagata
     2941 ccacgttctg tttctgggca cagatcgggg caccgtgcag aaggtcgtag tccttcctac
     3001 caatagctct gccagtgggg aactcatcct ggaggagctg gaagtcttca agaatcatgt
     3061 tcccataaca acaatgaaaa tctcatccaa aaagcaacag ttgtacgtga gctccaatga
     3121 gggggtttcc caagtctctc tgcatcgctg ccatatctac ggcacagcct gtgcggactg
     3181 ctgcttggcg agggatccat actgtgcctg ggatggccac tcttgctcta ggttctaccc
     3241 cactgggaag cggaggagcc gaagacaaga tgtgagacat ggaaacccac tgacacaatg
     3301 ccgagggttc aatctgaaag catacagaaa tgcagctgaa attgttcagt atggagtaag
     3361 aaataacagc actttccttg agtgtgctcc caagtctcca caggcatcta tcaagtggtt
     3421 gctgcagaaa gacaaagaca ggaggaagga ggttaaactg aacgagcgca ttatagctac
     3481 ttcccaagga ctactgattc gctctgttca agattctgac caaggactct accactgcat
     3541 tgccactgag aacagcttca aacagaccat agccaagatc aacttcaaag ttttagattc
     3601 agaaatggtg gccgttgtga cagacaagtg gtccccgtgg acatgggctg gctctgtgag
     3661 ggctctaccc ttccatccaa aggacatcct gggggcattc agccactcgg aaatgcagct
     3721 catcaatcag tactgcaaag acacccggca gcagcagcag ctgggggaag aaccacagaa
     3781 gatgagaggg gactatggca agctgaaggc tctcatcaac agcaggaaaa gcggcgcgcc
     3841 tgacaaaact cacacatgcc caccgtgccc agcacctgaa ctcctggggg gaccgtcagt
     3901 cttcctcttc cccccaaaac ccaaggacac cctcatgatc tcccggaccc ctgaggtcac
     3961 atgcgtggtg gtggacgtga gccacgaaga ccctgaggtc aagttcaact ggtacgtgga
     4021 cggcgtggag gtgcataatg ccaagacaaa gccgcgggag gagcagtaca acagcacgta
     4081 ccgtgtggtc agcgtcctca ccgtcctgca ccaggactgg ctgaatggca aggagtacaa
     4141 gtgcaaggtc tccaacaaag ccctcccagc ccccatcgag aaaaccatct ccaaagccaa
     4201 agggcagccc cgagaaccac aggtgtatac cctgccccca tcccgggatg agctgaccaa
     4261 gaaccaggtc agcctgacct gcctggtcaa aggcttctat cccagcgaca tcgccgtgga
     4321 gtgggagagc aatgggcagc cggagaacaa ctacaagacc acgcctcccg tgctggactc
     4381 cgacggctcc ttcttcctct acagcaagct caccgtggac aagagcaggt ggcagcaggg
     4441 gaacgtcttc tcatgctccg tgatgcatga ggctctgcac aaccactaca cgcagaagag
     4501 cctctccctg tctccgggta aaggcggcca tcatcaccat caccattaat ctagagatct
     4561 tattaaagca gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4621 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4681 tcaatgtatc ttatcatgtc tggtcgactc tagctagact cttccgcttc ctcgctcact
     4741 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     4801 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     4861 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     4921 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     4981 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     5041 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     5101 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     5161 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     5221 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     5281 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     5341 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     5401 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     5461 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     5521 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     5581 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     5641 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     5701 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     5761 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     5821 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     5881 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     5941 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     6001 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     6061 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     6121 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     6181 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     6241 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     6301 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     6361 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     6421 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     6481 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     6541 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     6601 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     6661 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     6721 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaggaatgt
     6781 gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt atgcaaagca
     6841 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
     6901 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
     6961 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7021 ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag
     7081 gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg
     7141 tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg
     7201 gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca tcagagcaga
     7261 ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta aggagaaaat
     7321 accgcatcag gaaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     7381 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     7441 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     7501 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     7561 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     7621 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     7681 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     7741 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcca ttcgccattc
     7801 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
     7861 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
     7921 cgacgttgta aaacgacggc cagtgaatt
//
