LOCUS       sequence-139962-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 
            cassette, and late SV40 pA signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 370 pTol2-lmo2:CherryDest
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     139..162
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    252..376
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(401..406,407..424,425..431)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(551..570)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
       61 actacgacgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      121 cctacaacgt caacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      181 aacagtacga gcgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaaga
      241 gatctcgaat cacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata
      301 tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac
      361 atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt atgcttccgg
      421 ctcgtataat gtgtggattt tgagttagga tccgtcgaga ttttcaggag ctaaggaagc
      481 taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat ggcatcgtaa
      541 agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga ccgttcagct
      601 ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt atccggcctt
      661 tattcacatt cttgcccgcc tgatgaatgc tcatccggaa tt
//
