LOCUS       sequence-140000-        1057 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SapTrap 3-site Destination vector for C-terminal GFP tagging with 
            embedded Cbr-unc-119 selectable marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Schwartz ML, Jorgensen EM
  TITLE     SapTrap, a Toolkit for High-Throughput CRISPR/Cas9 Gene Modification
            in Caenorhabditis elegans.
  JOURNAL   Genetics. 2016 Feb 2. pii: genetics.115.184275.
  PUBMED    26837755
REFERENCE   2  (bases 1 to 1057)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2016 Feb 2. pii: genetics.115.184275."
COMMENT     Sequence Label: pMLS235
FEATURES             Location/Qualifiers
     source          1..1057
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     promoter        234..732
                     /label=CeU6 promoter
                     /note="promoter for the C. elegans K09B11.12 U6 snRNA gene 
                     (Friedland et al., 2013)"
     misc_RNA        760..835
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcgtccaaga
      241 actcgtacaa aaatgctctg aagtaggtct cgagatcaat actgggaaga cgaaagtctt
      301 gcgaaaccga ttcgctgacc ccagtaaagt ctacttcggt agcccttccc ccaccaccca
      361 gctcgacgac gtcgacgagt acatctacct cggtcgtcaa atcaacgccc aaaacaactt
      421 gatgccggaa atccaccgaa gacgtcgagc agcctgggct gcattcaatg gaatcaagaa
      481 tgccaccgac tccatcaccg acaagaagat tcgtgcgaat ctgttcgact caattgtcct
      541 tccagcgctc acctacgttt cagaagcctg gacattcacc aaagctctat ccgaacgagt
      601 acgaatcaca catgcctccc ccacaaatta ttgatctaca tccccatttg gtatagtgtc
      661 ttacaggact ctcaatatgt cagctgcatg ataaatggtc cctatataat tgaattgcaa
      721 atctaaatgt ttgcgaagag cggatccatg ggctcttcgg ttttagagct agaaatagca
      781 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt
      841 ttcaataata ttctagatat ccctttttga aactaaaatg aaatactcta aatttttgag
      901 ttcaatgttt caactaatct tggttaaagg taaattcccg gagggacgat tttaaagttg
      961 ccagagttga ttaaattggt ccaaaaaatt ttattaggaa actcgaaaaa aaattgattt
     1021 tttttgacaa tattccaaag aaaattacaa aatctcg
//
