LOCUS       sequence-140011-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human C-terminal part (Q778-A1056) Plekhm1 in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Witwicka H, Jia H, Kutikov A, Reyes-Gutierrez P, Li X, Odgren PR
  TITLE     TRAFD1 (FLN29) Interacts with Plekhm1 and Regulates Osteoclast 
            Acidification and Resorption.
  JOURNAL   PLoS One. 2015 May 19;10(5):e0127537. doi: 
            10.1371/journal.pone.0127537. eCollection 2015.
  PUBMED    25992615
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-05-19"; volume: "10(5):e0127537. doi"; pages: " 
            10.1371/journal.pone.0127537. eCollection 2015"
COMMENT     Sequence Label: hPlekhm1-C/pIRES puro Glue (S778-A1056)
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     42..61
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        42..60
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             108..221
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             240..260
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             267..293
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             267..293
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             294..371
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
       61 gagacccaag cttggtaccg agctcggatc gatttaatta agccaccatg gacgagaaga
      121 ccaccggctg gcggggcggc cacgtggtgg agggcctggc cggcgagctg gagcagctgc
      181 gggcccggct ggagcaccac ccccagggcc agcgggagcc cggcggaagc cccggtggcg
      241 agaacctgta cttccagggc gcaagctacc cctacgacgt gcccgactac gccaagcggc
      301 ggtggaagaa gaacttcatc gccgtgagcg ccgccaaccg gttcaagaag atcagcagca
      361 gcggcgccct gattgatggg ggatctggcc ccggcggcgg cgcgcctgta cagatatcct
      421 cctacttgga gacagccgag gaggcggtga ccctgggcgg gagcctggat gaaaactgtc
      481 aggaggtgct gaaatttgcc acccgggaga atggcttcct gctgcagtac ctggtggcta
      541 tccccatgga gaaaggcctt gactcccaag gctgcttctg cgcaggctgc tcccggcaga
      601 tcggcttctc ctttgtacga cccaagctct gtgccttctc tggcctctat tactgtgaca
      661 tctgccacca agacgatgcc tcagtgattc cggccaggat catccacaac tgggacctca
      721 ccaagcgccc gatctgcagg caggccctga agtttctgac acagatccgg gcccagcccc
      781 tcatcaacct gcagatggtg aacgcgtctc tgtacgagca tgtggagcgg atgcacctca
      841 ttgggaggag acgggagcag ctgaagctcc tgggggatta cctgggcctg tgccgga
//
