LOCUS       sequence-140014-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Photoactivatable transcription system. Expression of genomic anchor 
            probe, containing dCas9 and CIB1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Nihongaki Y, Yamamoto S, Kawano F, Suzuki H, Sato M
  TITLE     CRISPR-Cas9-based Photoactivatable Transcription System.
  JOURNAL   Chem Biol. 2015 Feb 19;22(2):169-74. doi: 
            10.1016/j.chembiol.2014.12.011. Epub 2015 Jan 22.
  PUBMED    25619936
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.chembiol.2014.12.011"; journalName: "Chem Biol"; date: 
            "2015-02-19- 19"; volume: "22"; issue: "2"; pages: "169-74"
COMMENT     Sequence Label: NLS-dCas9-trCIB1
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    100..324
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     313..330
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtggggat
       61 accccctaga gccccagctg gttctttccg cctcagaagc catagagccc accgcatccc
      121 cagcatgcct gctattgtct tcccaatcct cccccttgct gtcctgcccc accccacccc
      181 ccagaataga atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa
      241 ggacagtggg agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag
      301 atggctggca actagaaggc acagtcgagg ctgatcagcg ggtttaaacg ggccctctag
      361 actcgaggcc tccgctgcca cttccagatc ccccgccgct ccccccagaa cccccagaac
      421 tggtattcac ttgctgcatt ggattgacat gaaggtaacc ggagttaacc atctcactag
      481 aataaccaga gtgaaccatc tcatgagaat aaccggaaag aaccatttca ggagatggca
      541 ccacagtcat tggagttgag gcaacctctt tggcaaaaat gtcatccata tcaaaatccg
      601 gccttggatt cacaattgct agtttcatcg ataagaactc gatttgtctc tgaagagact
      661 gaacatagtt aatgatttca tcaagcatcc ctgctttgcc tgtgatcttg tcgcatccag
      721 gaaccaaatc ttgtagaaac ttcattctct cactgatctt ttctcttcta actcgttctg
      781 ctatgctgtg actatcagtg gcttggcctc gtcgtgcacg aacatgaata taatccgttt
      841 tctccaattc cttcgtcact ttagatgaat cattagagaa attgttctct tctttcttgg
      901 ctttgtgttt catcttcttg atgctttttg tgctcccatt gttttgctct gttattttcg
      961 acttctcttc ttctccttct tctactaggt catctctgtt catcgtcatc
//
