LOCUS       sequence-140173-         976 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 (PH1 and PH2 domains) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 976)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1 -PH1/2 pGEX 4T2-GST
FEATURES             Location/Qualifiers
     source          1..976
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(865..882)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(909..931)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 cgcgcgaggc agatcgtcag tcagtcacga tgcggccgct cgaagtcact cggctgtctc
       61 caagtaggat gccaggactt tgcggaccag atccctccac agggcggctt cctcggcgtt
      121 tccggcctgc agcttcagga cagccttggc cgtgatgatt ttgaagaagg atgggccccc
      181 aaggctggtg tctggcagga tgtcccggat ggtctccacg ccgtggctgt cactcagcat
      241 cttctcattg ttcctgatgc ggaaacattt cagagcctcc aaggacagag aaaatatata
      301 gggcatccag gtcctgtcca tgtacaagta cagcagggac tccttgatgg catctggctc
      361 tggaacctgg gcggacgacc agtcaaactg tgtgccctgg agggccgcgg gctccgagag
      421 caggtctgag ggagagaggc agccctgggg cgcctcgggg ggttcctcag gctggtctgg
      481 gtactgcacg ttcacccact catcctcctg ctgaggccgg accttctgca gggcctcccg
      541 cacccggtcc agccagtcct cagcttcgtc ctgggaggag gcgcgcaggg ccagcttctt
      601 gccagagaag accagctcaa agcgcccatc actatgggct ggccccacag actcacagcg
      661 aagcagcgag cagttctcca cacaggtgtg ctcctcgttg ctcaggtaga ggcggaactc
      721 cagcggggag agctcgcaga agagctcctt ccagatgccc attgcccccc gccgctccac
      781 ggtgcccagc ttcatgagac cccggaatgg gttggacagt cccatctgtc tccggtgtac
      841 cacccggaag ctcttatgcc cctgggatcc acgcggaacc agatccgatt ttggaggatg
      901 gtcgccacca ccaaacgtgg cttgccagcc ctgcaaaggc catgctatat acttgctgga
      961 tttcaagtac ttatca
//
