LOCUS       sequence-140184-         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express human Plekhm1 (PH2 domain) in bacterial cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  TITLE     Plekhm1 pFastHFTb
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Plekhm1- PH2 pGEX 4T2-GST
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(531..553)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(698..716)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        784..888
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcggatctgg ttccgcgtgg atcccagtac ccagaccagc ctgaggaacc ccccgaggcg
       61 ccccagggct gcctctctcc ctcagacctg ctctcggagc ccgcggccct ccagggcaca
      121 cagtttgact ggtcgtccgc ccaggttcca gagccagatg ccatcaagga gtccctgctg
      181 tacttgtaca tggacaggac ctggatgccc tatatatttt ctctgtcctt ggaggctctg
      241 aaatgtttcc gcatcaggaa caatgagaag atgctgagtg acagccacgg cgtggagacc
      301 atccgggaca tcctgccaga caccagcctt gggggcccat ccttcttcaa aatcatcacg
      361 gccaaggctg tcctgaagct gcaggccgga aacgccgagg aagccgccct gtggagggat
      421 ctggtccgca aagtcctggc atcctacttg gagacagccg agtgactcga gcggccgcat
      481 cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
      541 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
      601 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt
      661 agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
      721 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      781 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      841 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      901 cattt
//
