LOCUS       sequence-140253-        9172 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9172)
  AUTHORS   Rahman S, Sowa ME, Ottinger M, Smith JA, Shi Y, Harper JW, Howley PM
  TITLE     The Brd4 Extraterminal Domain Confers Transcription Activation 
            Independent of pTEFb by Recruiting Multiple Proteins, Including 
            NSD3.
  JOURNAL   Mol Cell Biol. 2011 Jul . 31(13):2641-52.
  PUBMED    21555454
REFERENCE   2  (bases 1 to 9172)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2011 Jul . 31(13):2641-52."
COMMENT     Sequence Label: p6345 MSCV-CMV-Flag-HA-Brd4 1-722
FEATURES             Location/Qualifiers
     source          1..9172
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     100..119
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     313..335
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     463..482
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             682..1198
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1262..1603
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1670..2086
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     2016..2038
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     2054..2070
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        2101..2480
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2481..2684
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2635..2655
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2686..2704
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2707..2725
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2735..2759
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2832..2841
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2841..2864
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2877..2903
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    2919..2943
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2964..2984
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     protein_bind    complement(5176..5200)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        5219..5718
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5254..5277)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5651..5670
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5739..6338
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5739..5758)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6235..6255
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     protein_bind    6924..6940
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6948..6954,6955..6972,6973..6978))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6993..7014
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7131..7148)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7302..7890)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7382..7401)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8061..8852,8853..8921))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8684..8703
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8922..9026)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9094..9112
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9150..9172)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
       61 cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
      121 tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
      181 tcaggcgcca ttcgccattc aggctgcgca actgttggga agggcgatcg gtgcgggcct
      241 cttcgctatt acgccagctg gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa
      301 cgccagggtt ttcccagtca cgacgttgta aaacgacggc gcaaggaatg gtgcatgcaa
      361 ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca
      421 agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata
      481 ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag
      541 gcgattagtc caatttgtta aagacaggat atcagtggtc caggctctag ttttgactca
      601 acaatatcac cagctgaagc ctatagagta cgagccatag ataaaataaa agattttatt
      661 tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca agctagctta
      721 agtaacgcca ttttgcaagg catggaaaat acataactga gaatagagaa gttcagatca
      781 aggttaggaa cagagagaca gcagaatatg ggccaaacag gatatctgtg gtaagcagtt
      841 cctgccccgg ctcagggcca agaacagatg gtccccagat gcggtcccgc cctcagcagt
      901 ttctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct
      961 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag
     1021 ctcaataaaa gagcccacaa cccctcactc ggcgcgccag tcctccgata gactgcgtcg
     1081 cccgggtacc cgtattccca ataaagcctc ttgctgtttg catccgaatc gtggactcgc
     1141 tgatccttgg gagggtctcc tcagattgat tgactgccca cctcgggggt ctttcatttg
     1201 gaggttccac cgagatttgg agacccctgc ccagggacca ccgacccccc cgccgggagg
     1261 taagctggcc agcggtcgtt tcgtgtctgt ctctgtcttt gtgcgtgttt gtgccggcat
     1321 ctaatgtttg cgcctgcgtc tgtactagtt agctaactag ctctgtatct ggcggacccg
     1381 tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc ccagggactt
     1441 tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc cgaccccgtc
     1501 aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc cgtctgaatt
     1561 tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct gcagcatcgt
     1621 tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg gccagactgt
     1681 taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga tcgctcacaa
     1741 ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag aatggccaac
     1801 ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca cccaggttaa
     1861 gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct acatcgtgac
     1921 ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac accctaagcc
     1981 tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc gttcgacccc
     2041 gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggaa ttagatcctt
     2101 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     2161 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     2221 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     2281 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     2341 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     2401 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     2461 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     2521 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     2581 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     2641 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctccct atcagtgata
     2701 gagatctccc tatcagtgat agagatcgtc gacgagctcg tttagtgaac cgtcagatcg
     2761 cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc
     2821 tccggggatc tgccaccatg gactacaagg atgacgatga caagctcgat ggaggatacc
     2881 catacgatgt tccagattac gctgctcgag gttatcaaac aagtttgtac aaaaaagcag
     2941 gctccgcggc cgccgtactc gaggaaaacc tgtattttca gggctccttc acctctgcgg
     3001 agagcggccc tgggacgaga ttgagaaatc tgccagtaat gggggatgga ctagaaactt
     3061 cccaaatgtc tacaacacag gcccaggccc aaccccagcc agccaacgca gccagcacca
     3121 accccccgcc cccagagacc tccaacccta acaagcccaa gaggcagacc aaccaactgc
     3181 aatacctgct cagagtggtg ctcaagacac tatggaaaca ccagtttgca tggcctttcc
     3241 agcagcctgt ggatgccgtc aagctgaacc tccctgatta ctataagatc attaaaacgc
     3301 ctatggatat gggaacaata aagaagcgct tggaaaacaa ctattactgg aatgctcagg
     3361 aatgtatcca ggacttcaac actatgttta caaattgtta catctacaac aagcctggag
     3421 atgacatagt cttaatggca gaagctctgg aaaagctctt cttgcaaaaa ataaatgagc
     3481 tacccacaga agaaaccgag atcatgatag tccaggcaaa aggaagagga cgtgggagga
     3541 aagaaacagg gacagcaaaa cctggcgttt ccacggtacc aaacacaact caagcatcga
     3601 ctcctccgca gacccagacc cctcagccga atcctcctcc tgtgcaggcc acgcctcacc
     3661 ccttccctgc cgtcaccccg gacctcatcg tccagacccc tgtcatgaca gtggtgcctc
     3721 cccagccact gcagacgccc ccgccagtgc ccccccagcc acaaccccca cccgctccag
     3781 ctccccagcc cgtacagagc cacccaccca tcatcgcggc caccccacag cctgtgaaga
     3841 caaagaaggg agtgaagagg aaagcagaca ccaccacccc caccaccatt gaccccattc
     3901 acgagccacc ctcgctgccc ccggagccca agaccaccaa gctgggccag cggcgggaga
     3961 gcagccggcc tgtgaaacct ccaaagaagg acgtgcccga ctctcagcag cacccagcac
     4021 cagagaagag cagcaaggtc tcggagcagc tcaagtgctg cagcggcatc ctcaaggaga
     4081 tgtttgccaa gaagcacgcc gcctacgcct ggcccttcta caagcctgtg gacgtggagg
     4141 cactgggcct acacgactac tgtgacatca tcaagcaccc catggacatg agcacaatca
     4201 agtctaaact ggaggcccgt gagtaccgtg atgctcagga gtttggtgct gacgtccgat
     4261 tgatgttctc caactgctat aagtacaacc ctcctgacca tgaggtggtg gccatggccc
     4321 gcaagctcca ggatgtgttc gaaatgcgct ttgccaagat gccggacgag cctgaggagc
     4381 cagtggtggc cgtgtcctcc ccggcagtgc cccctcccac caaggttgtg gccccgccct
     4441 catccagcga cagcagcagc gatagctcct cggacagtga cagttcgact gatgactctg
     4501 aggaggagcg agcccagcgg ctggctgagc tccaggagca gctcaaagcc gtgcacgagc
     4561 agcttgcagc cctctctcag ccccagcaga acaaaccaaa gaaaaaggag aaagacaaga
     4621 aggaaaagaa aaaagaaaag cacaaaagga aagaggaagt ggaagagaat aaaaaaagca
     4681 aagccaagga acctcctcct aaaaagacga agaaaaataa tagcagcaac agcaatgtga
     4741 gcaagaagga gccagcgccc atgaagagca agccccctcc cacgtatgag tcggaggaag
     4801 aggacaagtg caagcctatg tcctatgagg agaagcggca gctcagcttg gacatcaaca
     4861 agctccccgg cgagaagctg ggccgcgtgg tgcacatcat ccagtcacgg gagccctccc
     4921 tgaagaattc caaccccgac gagattgaaa tcgactttga gaccctgaag ccgtccacac
     4981 tgcgtgagct ggagcgctat gtcacctcct gtttgcggaa gaaaaggaaa cctcaagctg
     5041 agaaagttga tgtgattgcc ggctcctcca agatgaaggg cttctcgtcc tcagagtcgg
     5101 agagctccag tgagtccagc tcctctgaca gcgaagactc cgaaacaggt cctgcctaaa
     5161 agggtgggcg cgccgaccca gctttcttgt acaaagtggt tcgataacga attctaccgg
     5221 gtaggggagg cgcttttccc aaggcagtct ggagcatgcg ctttagcagc cccgctgggc
     5281 acttggcgct acacaagtgg cctctggcct cgcacacatt ccacatccac cggtaggcgc
     5341 caaccggctc cgttctttgg tggccccttc gcgccacctt ctactcctcc cctagtcagg
     5401 aagttccccc ccgccccgca gctcgcgtcg tgcaggacgt gacaaatgga agtagcacgt
     5461 ctcactagtc tcgtgcagat ggacagcacc gctgagcaat ggaagcgggt aggcctttgg
     5521 ggcagcggcc aatagcagct ttgctccttc gctttctggg ctcagaggct gggaaggggt
     5581 gggtccgggg gcgggctcag gggcgggctc aggggcgggg cgggcgcccg aaggtcctcc
     5641 ggaggcccgg cattctgcac gcttcaaaag cgcacgtctg ccgcgctgtt ctcctcttcc
     5701 tcatctccgg gcctttcgac ctgcagccca agcttaccat gaccgagtac aagcccacgg
     5761 tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg caccctcgcc gccgcgttcg
     5821 ccgactaccc cgccacgcgc cacaccgtcg atccggaccg ccacatcgag cgggtcaccg
     5881 agctgcaaga actcttcctc acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg
     5941 acgacggcgc cgcggtggcg gtctggacca cgccggagag cgtcgaagcg ggggcggtgt
     6001 tcgccgagat cggcccgcgc atggccgagt tgagcggttc ccggctggcc gcgcagcaac
     6061 agatggaagg cctcctggcg ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg
     6121 tcggcgtctc gcccgaccac cagggcaagg gtctgggcag cgccgtcgtg ctccccggag
     6181 tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc
     6241 tccccttcta cgagcggctc ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac
     6301 cgcgcacctg gtgcatgacc cgcaagcccg gtgcctgacg cccgccccac gacccgcagc
     6361 gcccgaccga aaggagcgca cgaccccatg catcgataaa ataaaagatt ttatttagtc
     6421 tccagaaaaa ggggggaatg aaagacccca cctgtaggtt tggcaagcta gagaaccatc
     6481 agatgtttcc agggtgcccc aaggacctga aatgaccctg tgccttattt gaactaacca
     6541 atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc
     6601 cacaacccct cactcggcgc gccagtcctc cgatagactg cgtcgcccgg gtacccgtgt
     6661 atccaataaa ccctcttgca gttgcatccg acttgtggtc tcgctgttcc ttgggagggt
     6721 ctcctctgag tgattgacta cccgtcagcg ggggtctttc atgggtaaca gtttcttgaa
     6781 gttggagaac aacattctga gggtaggagt cgaatattaa gtaatcctga ctcaattagc
     6841 cactgttttg aatccacata ctccaatact cctgaaatag ttcattatgg acagcgcaga
     6901 aagagctggg gagaattgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     6961 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7021 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7081 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7141 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7201 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7261 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7321 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7381 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7441 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     7501 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     7561 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     7621 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     7681 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     7741 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     7801 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     7861 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     7921 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     7981 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8041 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8101 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8161 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8221 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8281 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8341 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8401 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     8461 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     8521 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     8581 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     8641 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     8701 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     8761 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8821 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8881 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8941 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9001 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa
     9061 gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt
     9121 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc gg
//
