LOCUS       sequence-140349-         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 263 
            of Arrestin..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash5
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    570..691
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(607..626)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     661..680
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(835..856)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attctgcaag gtgtacacca taaccccgct gctcagtgac aaccgagaga agcgtggcct
       61 tgcccttgat gggcaactca agcacgaaga caccaacctg gcttccagca ccattgtgaa
      121 ggagggagcc aacaaggagg tgctgggaat cctagtatcc tacagggtca aggtgaagct
      181 ggtggtgtct cgaggcgggg atgtctccgt ggagctacct ttcgtcctaa tgcaccccaa
      241 gccccacgac cacatcaccc ttccccgacc ccagtcagcc ccccgggaaa tagacatccc
      301 tgtggatacc aacctcattg aattcgatac caactatgcc acagacgacg acatcgtgtt
      361 tgaggacttt gcgaggcttc ggctgaaggg gatgaaggat gacgactgtg atgaccagtt
      421 ctgctagtct agagggcccg ggatccaccg gatctagata actgatcata atcagccata
      481 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
      541 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
      601 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
      661 gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg
      721 ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc
      781 ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt
      841 tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc
      901 tatcagggcg atggcccact acgtgaacca tcaccctaat caag
//
