LOCUS       sequence-140361-         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 410 
            of Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash6
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             417..434
                     /codon_start=1
                     /product="tetracysteine peptide that binds biarsenical 
                     labeling reagents"
                     /label=tetracysteine tag
                     /translation="CCPGCC"
     polyA_signal    580..701
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(617..636)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     671..690
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(845..866)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aggtgtacac cataaccccg ctgctcagtg acaaccgaga gaagcgtggc cttgcccttg
       61 atgggcaact caagcacgaa gacaccaacc tggcttccag caccattgtg aaggagggag
      121 ccaacaagga ggtgctggga atcctagtat cctacagggt caaggtgaag ctggtggtgt
      181 ctcgaggcgg ggatgtctcc gtggagctac ctttcgtcct aatgcacccc aagccccacg
      241 accacatcac ccttccccga ccccagtcag ccccccggga aatagacatc cctgtggata
      301 ccaacctcat tgaattcgat accaactatg ccacagacga cgacatcgtg tttgaggact
      361 ttgcgaggct tcggctgaag gggatgaagg atgacgactg tgatgaccag ttctgctgtt
      421 gtcctggttg ttgttagtct agagggcccg ggatccaccg gatctagata actgatcata
      481 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
      541 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
      601 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
      661 cattctagtt gtggtttgtc caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt
      721 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
      781 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
      841 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
      901 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg
      961 gggtcgaggt gccgtaaagc actaaatcg
//
