LOCUS       sequence-140537-         866 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Entry vector for Bioparts - Oligo anneal or PCR with internal BsaI 
            sites at 5' and 3' ends using NdeI/SphI cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 866)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 866)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-lacZ
FEATURES             Location/Qualifiers
     source          1..866
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(19..62)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    65..84
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     protein_bind    103..119
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     108..130
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     127..143
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     127..143
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     RBS             152..163
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      516..573
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(597..614)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
       61 tctggaattc gcggccgctt ctagaatcta gagacgcata tgttgtgagc ggataacaat
      121 ttcacacagg aaacagctat gaccatggta caaagaggag aaaggacatg ggtgacccga
      181 aactgctgct ggaagttccg cacgctatct cttcttctcc gggtaactct ctggctgttg
      241 ttctgcaacg tcgtgactgg gaaaacccgg gtgttaccca gctgaaccgt ctggctgctc
      301 acccgccgtt cgcttcttgg cgtaactctg aagaagctcg taccgaccgt ccgtcccagc
      361 agctgcgttc tctgatccgt ctgctgacca aaccggaacg taaactgtct tggctgctgc
      421 cgccgctgtc taacaactaa cgcaaaaaac cccgcccctg acagggcggg gttttttcgc
      481 actagtggat ccgcatgccg tctcattagc tgcagtccgg caaaaaaggg caaggtgtca
      541 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttatgca ggcttcctcg
      601 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
      661 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
      721 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccacaggctc
      781 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
      841 ggactataaa gataccaggc gtttcc
//
