LOCUS       sequence-140602-         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Provides the O. sativa U3 RNA polIII promoter as a level 0 
            GoldenBraid part.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pUPD_OsU3 (GB1184)
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(51..431)
                     /label=OsU3 promoter
                     /note="Oryza sativa (rice) snRNA U3 promoter"
     promoter        complement(534..552)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(535..552)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(570..586)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(570..586)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(583..605)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    594..610
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(618..624,625..642,643..648))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    663..684
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(801..818)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ccggccgcca tggcggccgc gggaattcga ttgggcgatg agtggtctcg tgccacggat
       61 catctgcaca actcttttaa atcagctttg atctatgtgg atagccgagg tggtactaat
      121 actagtcttt gttgtcgtcc aattgcgtaa tgggccggcc catactgcaa tacatgtcct
      181 gaaaggcttc atggcccact acgaaatgct tttctcctac agtttatctt actccttcac
      241 atcacgtggt ttccaacgta cccagtgttc ccggcttcca gcatttgctg gtagcaccag
      301 tagaagacgc ctgtcttgtg ctatggtccc tgactgcaca tctgattcct ccaagatcca
      361 tgcatgcctg ataactttaa gttgcttcag aagaacttta agtgatctgt tcgtatgttt
      421 aaagattcct tctcccgaga ccactcatcg cccaatcact agtgaattcg cggccgcctg
      481 caggtcgacc atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag
      541 tgtcacctaa atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
      601 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
      661 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
      721 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
      781 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
      841 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggg
//
