LOCUS       sequence-140620-         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L3S2P21 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L3S2P21
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    218..237
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      240..283
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(287..305)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(533..552)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacagga ccaaaacgaa aaaaggcccc cctttcggga ggcctctttt ctggaatttg
      181 gtaccgagtc gatgagaccc atatgcgtct ctagattcta gaagcggccg cgaattccag
      241 aaatcatcct tagcgaaagc taaggatttt ttttatctga aattctgcct cgtgatacgc
      301 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactcga
      361 gttgatcggg cacgtaagag gttccaactt tcaccataat gaaataagat cactaccggg
      421 cgtatttttt gagttatcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat
      481 cactggatat accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt
      541 tcagtcagtt gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt
      601 aaagaccgta aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg
      661 cctgatgaat gctcatccgg aatttcgtat ggcaatgaaa gacggtgagc tggtgatatg
      721 ggatagtgtt cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct
      781 ctggagtga
//
