LOCUS       sequence-140629-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  L2U5H08 terminator.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-L2U5H08
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(36..93)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     misc_feature    187..206
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      209..252
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(256..274)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(502..521)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 tcagtgagcg aggaagcctg cataacgcga agtaatcttt tcggttttaa agaaaaaggg
       61 cagggtggtg acaccttgcc cttttttgcc ggactgcagc taatgagacg gcatgcggtc
      121 tctaacaata caacaatgcc gggatgttct cccggcatcg atgagaccca tatgcgtctc
      181 tagattctag aagcggccgc gaattccaga aatcatcctt agcgaaagct aaggattttt
      241 tttatctgaa attctgcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      301 tggtttctta gacgtcaggt ggcactcgag ttgatcgggc acgtaagagg ttccaacttt
      361 caccataatg aaataagatc actaccgggc gtattttttg agttatcgag attttcagga
      421 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
      481 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
      541 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
      601 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga atttcgtatg
      661 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
      721 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
      781 tttctacaca tatattcgca
//
