LOCUS       sequence-140636-        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Backbone for expressing plant artificial miRNAs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Schwab R, Ossowski S, Riester M, Warthmann N, Weigel D
  TITLE     Highly specific gene silencing by artificial microRNAs in 
            Arabidopsis.
  JOURNAL   Plant Cell. 2006 May . 18(5):1121-33.
  PUBMED    16531494
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Cell.
            2006 May . 18(5):1121-33."
COMMENT     Sequence Label: pRS300
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     86..108
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     100..117
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     101..117
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..143
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        124..142
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     167..183
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     168..184
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(622..638)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(622..638)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     promoter        complement(675..693)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(714..730)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(714..730)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(727..749)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    738..754
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(762..768,769..786,787..792))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    807..828
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(945..962)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
       61 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa
      121 ttgtaatacg actcactata gggcgaattg ggtaccgggc cccccctcga ggtcgacggt
      181 atcgataagc ttgatatcga attcctgcag ccccaaacac acgctcggac gcatattaca
      241 catgttcata cacttaatac tcgctgtttt gaattgatgt tttaggaata tatatgtaga
      301 gagagcttcc ttgagtccat tcacaggtcg tgatatgatt caattagctt ccgactcatt
      361 catccaaata ccgagtcgcc aaaattcaaa ctagactcgt taaatgaatg aatgatgcgg
      421 tagacaaatt ggatcattga ttctctttga ttggactgaa gggagctccc tctctctttt
      481 gtattccaat tttcttgatt aatctttcct gcacaaaaac atgcttgatc cactaagtga
      541 catatatgct gccttcgtat atatagttct ggtaaaatta acattttggg tttatcttta
      601 tttaaggcat cgccatgggg ggatccacta gttctagagc ggccgccacc gcggtggagc
      661 tccagctttt gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag
      721 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
      781 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
      841 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
      901 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
      961 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
//
