LOCUS       sequence-140652-         828 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of several 
            non-standard amino acids, into proteins in E coli in response to the
            amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 828)
  AUTHORS   Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW,
            Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll 
            D, Rinehart J, Isaacs FJ
  TITLE     Evolution of translation machinery in recoded bacteria enables 
            multi-site incorporation of nonstandard amino acids.
  JOURNAL   Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. 
            Epub 2015 Nov 16.
  PUBMED    26571098
REFERENCE   2  (bases 1 to 828)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3372"; 
            journalName: "Nat Biotechnol"; date: "2015-12"; volume: "33"; issue:
            "12"; pages: "1272-1279"
COMMENT     Sequence Label: pEvol-pAcFRS.2.t1
FEATURES             Location/Qualifiers
     source          1..828
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cgcaactctc tactgtttct ccatacccgt ttttttgggc taacaggagg aattagatct
       61 atggacgaat ttgaaatgat aaagagaaac acatctgaaa ttatcagcga ggaagagtta
      121 agagaggttt taaaaaaaga tgaaaaatct gctctgatag gttttgaacc aagtggtaaa
      181 atacatttag ggcattatct ccaaataaaa aagatgattg atttacaaaa tgctggattt
      241 gatataatta tagttttggc tgatttacac gcctatttaa accagaaagg agagttggat
      301 gagattagaa aaataggaga ttataacaaa aaagtttttg aagcaatggg gttaaaggca
      361 aaatatgttt atggaagtga attccagctt gataaggatt atacactgaa tgtctataga
      421 ttggctttaa aaactacctt aaaaagagca agaaggagta tggaacttat agcaagagag
      481 gatgaaaatc caaaggttgc tgaagttatc tatccaataa tgcaggttaa tggttgtcat
      541 tataggggcg ttgatgttga tgttggaggg atggagcaga gaaaaataca catgttagca
      601 agggagcttt taccaaaaaa ggttgtttgt attcacaacc ctgtcttaac gggtttggat
      661 ggagaaggaa agatgagttc ttcaaaaggg aattttatag ctgttgatga ctctccagaa
      721 gagattaggg ctaagataaa gaaagcatac tgcccagctg gagttgttga aggaaatcca
      781 ataatggaga tagctaaata cttccttgaa tatcctttaa ccataaaa
//
