LOCUS       sequence-140670-         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSIREN-RetroQ vector containing shRNA sequence to BPTF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  TITLE     BPTF shRNA Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pSIREN-RetroQ-BPTF-sh28
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     122..141
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     promoter        269..768
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(304..327)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     701..720
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(789..808)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 tgttagagag ataattagaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
       61 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
      121 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
      181 gtggaaagga cgaggatcca gcacagagaa gaccatgatt caagagatca tggtcttctc
      241 tgtgcttttt ttacgcgtga attctaccgg gtaggggagg cgcttttccc aaggcagtct
      301 ggagcatgcg ctttagcagc cccgctgggc acttggcgct acacaagtgg cctctggcct
      361 cgcacacatt ccacatccac cggtaggcgc caaccggctc cgttctttgg tggccccttc
      421 gcgccacctt ctactcctcc cctagtcagg aagttccccc ccgccccgca gctcgcgtcg
      481 tgcaggacgt gacaaatgga agtagcacgt ctcactagtc tcgtgcagat ggacagcacc
      541 gctgagcaat ggaagcgggt aggcctttgg ggcagcggcc aatagcagct ttgctccttc
      601 gctttctggg ctcagaggct gggaaggggt gggtccgggg gcgggctcag gggcgggctc
      661 aggggcgggg cgggcgcccg aaggtcctcc ggaggcccgg cattctgcac gcttcaaaag
      721 cgcacgtctg ccgcgctgtt ctcctcttcc tcatctccgg gcctttcgac ctgcagccca
      781 agcttaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca
      841 gggccgtacg caccc
//
