LOCUS       sequence-140675-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 promoter and RBS  - derived from pET3a (5' CTAT/3' CATA) fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-T7_RBS
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(21..64)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    67..86
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     primer_bind     126..145
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        126..144
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    145..169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             184..206
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     terminator      246..303
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     primer_bind     complement(327..344)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(578..597)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 taggcgtatc acgaggcaga atttcagata aaaaaaatcc ttagctttcg ctaaggatga
       61 tttctggaat tcgcggccgc ttctagaatc tagagacgca tatgggtctc actatcccgc
      121 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ctctagaaat
      181 aattttgttt aactttaaga aggagatata ccataagaga ccgcatgccg tctcattagc
      241 tgcagtccgg caaaaaaggg caaggtgtca ccaccctgcc ctttttcttt aaaaccgaaa
      301 agattacttc gcgttatgca ggcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
      361 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
      421 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
      481 gccgcgttgc tggcgttttt ccacaggctc cgcccccctg acgagcatca caaaaatcga
      541 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
      601 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
      661 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
      721 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
      781 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
      841 ctggcagcag ccactggta
//
