LOCUS       sequence-140689-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Sheen JH, Zoncu R, Kim D, Sabatini DM
  TITLE     Defective Regulation of Autophagy upon Leucine Deprivation Reveals a
            Targetable Liability of Human Melanoma Cells In Vitro and In Vivo.
  JOURNAL   Cancer Cell. 2011 May 17. 19(5):613-28.
  PUBMED    21575862
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Cell. 2011 May 17. 19(5):613-28."
COMMENT     Sequence Label: pQCXI Neo DsRed-LC3-GFP
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      97..106
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..777
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Strongin et al., 2007)"
                     /label=DsRed-Monomer
                     /note="mammalian codon-optimized"
                     /translation="MDNTEDVIKEFMQFKVRMEGSVNGHYFEIEGEGEGKPYEGTQTAK
                     LQVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYMKLSFPEGFTWERSMNFEDGGV
                     VEVQQDSSLQDGTFIYKVKFKGVNFPADGPVMQKKTAGWEPSTEKLYPQDGVLKGEISH
                     ALKLKDGGHYTCDFKTVYKAKKPVQLPGNHYVDSKLDITNHNEDYTVVEQYEHAEARHS
                     GSQ"
     primer_bind     501..520
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tttgacctcc atagaagaca ccgggaccga tccagcctcc gcggccggga acggtgcatt
       61 ggaacggacc tgcaggaatt gatccgcggc cgctgagcca ccatggacaa caccgaggac
      121 gtcatcaagg agttcatgca gttcaaggtg cgcatggagg gctccgtgaa cggccactac
      181 ttcgagatcg agggcgaggg cgagggcaag ccctacgagg gcacccagac cgccaagctg
      241 caggtgacca agggcggccc cctgcccttc gcctgggaca tcctgtcccc ccagttccag
      301 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactacat gaagctgtcc
      361 ttccccgagg gcttcacctg ggagcgctcc atgaacttcg aggacggcgg cgtggtggag
      421 gtgcagcagg actcctccct gcaggacggc accttcatct acaaggtgaa gttcaagggc
      481 gtgaacttcc ccgccgacgg ccccgtaatg cagaagaaga ctgccggctg ggagccctcc
      541 accgagaagc tgtaccccca ggacggcgtg ctgaagggcg agatctccca cgccctgaag
      601 ctgaaggacg gcggccacta cacctgcgac ttcaagaccg tgtacaaggc caagaagccc
      661 gtgcagctgc ccggcaacca ctacgtggac tccaagctgg acatcaccaa ccacaacgag
      721 gactacaccg tggtggagca gtacgagcac gccgaggccc gccactccgg ctcccagtcc
      781 ggactcagat ctcgagctca agcttcgaat tctggtggtg gtccgtccga gaagaccttc
      841 aaacagcgcc ggagcttcga acaaagagtg gaagatgtcc ggctcatccg ggagcagcac
      901 cccaccaaga tcccagtgat tatagagcga tacaagggtg agaagcagct gcccgtcctg
      961 ga
//
