LOCUS       sequence-140731-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin3
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             55..78
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actcccaggt ccaactgcac ctcggttcta tcgattgaat tcaccatgga aatggactac
       61 aaggatgacg atgacaaggg tggtggtggt ggtgcgtcga cgatgaatcg gggggggggg
      121 tcaccatccg ccgccgccaa ctatctgctg tgcactaact gtagaaaagt cctgagaaaa
      181 gacaaacgga tccgcgtgag tcagccactg acacgaggcc ccagcgcctt cattcctgag
      241 aaggaagtgg tccaggctaa caccgtggat gagagaacaa attttctggt cgaggaatac
      301 agcacttccg ggaggctgga caacatcact caggtcatga gcctgcatac ccagtacctg
      361 gagtcattcc tgcggagcca gttttatatg ctgagaatgg atggacccct gcctctgcca
      421 taccgacact atatcgccat tatggccgct gcacggcacc agtgcagcta cctgatcaat
      481 atgcatgtgg acgagttcct gaagacaggc gggattgccg agtggctgaa cggcctggaa
      541 tatgtgcctc agcggctgaa gaacctgaat gagatcaaca agctgctggc ccacagacca
      601 tggctgatca ccaaagaaca tattcagaag ctggtgaaaa caggggagaa caattggtcc
      661 ctgcccgaac tggtccacgc agtggtcctg ctggcccact accatgccct ggctagtttc
      721 gtgtttggat caggcatcaa ccccgagagg gatcctgaaa tctcaaatgg cttccgcctg
      781 attagcgtga acaatttttg cgtctgtgac ctggctaacg ataacaatat cgagaatgca
      841 tctctg
//
