LOCUS       sequence-140732-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  overexpression, codon optimized for mammalian expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Chantranupong L, Wolfson RL, Orozco JM, Saxton RA, Scaria SM, 
            Bar-Peled L, Spooner E, Isasa M, Gygi SP, Sabatini DM
  TITLE     The Sestrins interact with GATOR2 to negatively regulate the 
            amino-acid-sensing pathway upstream of mTORC1.
  JOURNAL   Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1016/j.celrep.2014.09.014. 
            Epub 2014 Sep 25.
  PUBMED    25263562
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2014.09.014"; journalName: "Cell Rep"; date: 
            "2014-10-9- 9"; volume: "9"; issue: "1"; pages: "1-8"
COMMENT     Sequence Label: pRK5-FLAG-Sestrin3
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      6..15
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttgggccatg gcggccaagc tggggatcct tacttactta gcggccgcgt taggtcagat
       61 ggcgagtaat agccctcaga gcatacagca gttcagcctg cattcttgct tccatcagca
      121 gcaggttgac atgcactttc tcagagtgct taaactgccg ccaataggag tcgtacatcc
      181 ttttggtagt tctctcggga taacaagtga ctgtcttgat gtacaccttc agagatcttt
      241 ccagcagctg attcacttcg ccataatcgt aatcgtcata ccggattcca aacatgcagt
      301 ggacgtagtt gaacagtgcg cgcctcagca ttgtggtatc cacgtcttca tgagtagcca
      361 ttgtattata ggtcaggttg tagaccatgc ggaacttctc atccagcagg tgtccaatgt
      421 cagaatacag cctattcacc agggaaaacc catggttctc ccaagtgtag tcctgggcgc
      481 ggaatgtggg caggtgttcc tcgcctctcc gtgcaaagtc ttcatatccg aaccctggat
      541 cctcgatgta cctactcacg tcagaggtaa tgatcatatc gtcttcaaag tcagaatgag
      601 gaaaggagtg gaaagtatcg ccactgacca caaacagaga ctctttcttt tctttctcga
      661 atcgggtgga catttcctcc tggctagctt cctcgtcttc gcgttcctcc tgcagccgct
      721 tcattcgctc catcagggct tccagctcgg acaggctatc cacaatcccg aagtttgagc
      781 cactcagaga tgcattctcg atattgttat cgttagccag gtcacagacg caaaaattgt
      841 tcacgctaat caggcggaag ccatttgaga tttc
//
