LOCUS       sequence-140798-        1032 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expressing mIFP and EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Yu D, Baird MA, Allen JR, Howe ES, Klassen MP, Reade A, Makhijani K,
            Song Y, Liu S, Murthy Z, Zhang SQ, Weiner OD, Kornberg TB, Jan YN, 
            Davidson MW, Shu X
  TITLE     A naturally monomeric infrared fluorescent protein for protein 
            labeling in vivo.
  JOURNAL   Nat Methods. 2015 Aug;12(8):763-765. doi: 10.1038/nmeth.3447. Epub 
            2015 Jun 22.
  PUBMED    26098020
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3447"; journalName: "Nat Methods"; date: "2015-08"; 
            volume: "12"; issue: "8"; pages: "763-765"
COMMENT     Sequence Label: pLenti CMV_mIFP IRES EGFP
FEATURES             Location/Qualifiers
     source          1..1032
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             189..200
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             192..245
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgggcgttgg cgcgaccctg gtttgctctc tgatggtgtc tggtcgtctg tggggtctga
       61 tcgcttgcca ccactacgaa ccgcgcttcg ttccgttcca cattcgcgct gctggcgaag
      121 cgctggcgga aacttgtgcg atccgcatcg cgacgctgga gagctttgca cagtctcagt
      181 ccaaagatat cgagggcaga ggaagtctgc taacatgcgg tgacgtcgag gagaatcctg
      241 gcccaatgaa tatcgaagag ttcttcgagc tgagtgccgg gaagtggttt tcccaccgga
      301 cttcccacca cctggctttt aagcagtccg aagacggcaa gtctgatctg gtgatagaga
      361 gtctggccgc agatcacccc gaggtgatca aactctgtga gctgtacgag gtgcctgctt
      421 cagccgcaag ttgcggcgca agagtttcct ggaacggcac catggaatgg gatgaggaga
      481 aacatacagg gtccaccgtg ctggctaccg tgcccgacgt ggataacccc aacgaaggca
      541 ggctcctgcg cgagatgggt tacgctgaga aagctcccgt ggcaggccgc tacaaaatgg
      601 gcgacgatgg agcactgact ctgaccacag aatacgagac catgtggtca gaagaacggc
      661 tgtggttcgc aagccccaac ctccgcatgc gggtctccgt tctcaagagg ttcggcgggt
      721 tctcaatggc cagctttacc agtgagatac ggatgggagg atccccagcc gcagccaaag
      781 cagaggaagc cgccaatagc gcatcctcat gactcgagta ctccggtatt gcggtaccct
      841 tgtacgcctg ttttatactc ccttcccgta acttagacgc acaaaaccaa gttcaataga
      901 agggggtaca aaccagtacc accacgaaca agcacttctg tttccccggt gatgtcgtat
      961 agactgcttg cgtggttgaa agcgacggat ccgttatccg cttatgtact tcgagaagcc
     1021 cagtaccacc tc
//
