LOCUS       sequence-14082-e        5511 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5511)
  AUTHORS   Drews C, Senkel S, Ryffel GU
  TITLE     The nephrogenic potential of the transcription factors osr1, osr2, 
            hnf1b, lhx1 and pax8 assessed in Xenopus animal caps.
  JOURNAL   BMC Dev Biol. 2011 Jan 31;11:5. doi: 10.1186/1471-213X-11-5.
  PUBMED    21281489
REFERENCE   2  (bases 1 to 5511)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2011-01-31"; volume: "11:5. doi"; pages: " 
            10.1186/1471-213X-11-5"
COMMENT     Sequence Label: Ex1(-120/+3)
FEATURES             Location/Qualifiers
     source          1..5511
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(688..699)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      775..784
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             781..2433
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(847..865)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2474..2595
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2485..2504)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2539..2558
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2843..2860)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3014..3602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3094..3113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3773..4564,4565..4633))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4396..4415
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4634..4738)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4765..5220
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4852..4871)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5062..5083
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5351..5399
                     /note="synthetic polyadenylation signal"
     misc_feature    5413..5504
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5453..5472
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagtta atggtttacc agggactgcg
       61 aggcgctgat tggccgtggc agcccgtgac gtaacggcgt tgtgcataag gcttgctcct
      121 ctgctgttga gaacatgcag tccaggcgcg acagttcgga tgggaagagc aagtgagtgg
      181 agcaaacctg taggatcgcc agctccagtg ccaactactc aacaaccttc acagcagcca
      241 agggatcgca ctctgcattg gcactgtata tgtgcaaggg gcattccttc atatatggac
      301 tttgctcagc tgctagtggg atttggacaa tacagccagg gccagaagtt gttgatggac
      361 taggggactt ccttggtgac ataaagtgag aacgggcaaa ggaaccaccc ccggatacca
      421 aaaggagaaa gaaggaaaat aatctgccac ccaaagcagc gacccgagtt gtgcttgcga
      481 ggagcctgtc cgtagcactg gacgtgatgt cgggcaatcg aaatcccatt ggcttgtaat
      541 atatctctag gtaaatagat gcactttcag cagcgactgc gctacctcaa ctgatcggcc
      601 ctcacatctc tgtcctctcc tacctcctgc taccctggct tggggatctc catcagctcc
      661 gtcttcttgt ctttctagac cacagtgtct gccttctatt ctcctaatcc gcccattcct
      721 ctaaaatccc aaataaccaa agggaaagct tggcattccg gtactgttgg taaagccacc
      781 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
      841 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      901 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      961 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
     1021 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
     1081 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
     1141 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
     1201 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
     1261 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
     1321 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
     1381 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
     1441 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
     1501 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
     1561 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
     1621 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
     1681 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
     1741 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1801 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1861 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1921 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1981 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     2041 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     2101 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     2161 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     2221 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     2281 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     2341 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     2401 aaggccaaga agggcggaaa gatcgccgtg taattctaga gtcggggcgg ccggccgctt
     2461 cgagcagaca tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga
     2521 aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc
     2581 tgcaataaac aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag
     2641 gtgtgggagg ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat
     2701 ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     2761 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     2821 gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     2881 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     2941 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     3001 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     3061 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     3121 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     3181 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     3241 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     3301 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     3361 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     3421 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     3481 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     3541 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     3601 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     3661 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     3721 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     3781 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     3841 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     3901 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     3961 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     4021 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     4081 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     4141 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     4201 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     4261 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     4321 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     4381 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     4441 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     4501 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     4561 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     4621 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     4681 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     4741 catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4801 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     4861 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4921 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4981 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     5041 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     5101 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     5161 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt
     5221 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     5281 tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgg gaggtacttg
     5341 gagcggccgc aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga
     5401 atcgatagta ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa
     5461 ataggctgtc cccagtgcaa gtgcaggtgc cagaacattt ctctatcgat a
//
