LOCUS       sequence-14083-e        5506 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Drews C, Senkel S, Ryffel GU
  TITLE     The nephrogenic potential of the transcription factors osr1, osr2, 
            hnf1b, lhx1 and pax8 assessed in Xenopus animal caps.
  JOURNAL   BMC Dev Biol. 2011 Jan 31;11:5. doi: 10.1186/1471-213X-11-5.
  PUBMED    21281489
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2011-01-31"; volume: "11:5. doi"; pages: " 
            10.1186/1471-213X-11-5"
COMMENT     Sequence Label: Ex1(-117/+3)
FEATURES             Location/Qualifiers
     source          1..5506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(683..694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     regulatory      770..779
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             776..2428
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(842..860)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     polyA_signal    2469..2590
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2480..2499)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2534..2553
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2838..2855)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3009..3597)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3089..3108)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3768..4559,4560..4628))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4391..4410
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4629..4733)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      4760..5215
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4847..4866)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5057..5078
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    5346..5394
                     /note="synthetic polyadenylation signal"
     misc_feature    5408..5499
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     5448..5467
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagggt ttaccaggga ctgcgaggcg
       61 ctgattggcc gtggcagccc gtgacgtaac ggcgttgtgc ataaggcttg ctcctctgct
      121 gttgagaaca tgcagtccag gcgcgacagt tcggatggga agagcaagtg agtggagcaa
      181 acctgtagga tcgccagctc cagtgccaac tactcaacaa ccttcacagc agccaaggga
      241 tcgcactctg cattggcact gtatatgtgc aaggggcatt ccttcatata tggactttgc
      301 tcagctgcta gtgggatttg gacaatacag ccagggccag aagttgttga tggactaggg
      361 gacttccttg gtgacataaa gtgagaacgg gcaaaggaac cacccccgga taccaaaagg
      421 agaaagaagg aaaataatct gccacccaaa gcagcgaccc gagttgtgct tgcgaggagc
      481 ctgtccgtag cactggacgt gatgtcgggc aatcgaaatc ccattggctt gtaatatatc
      541 tctaggtaaa tagatgcact ttcagcagcg actgcgctac ctcaactgat cggccctcac
      601 atctctgtcc tctcctacct cctgctaccc tggcttgggg atctccatca gctccgtctt
      661 cttgtctttc tagaccacag tgtctgcctt ctattctcct aatccgccca ttcctctaaa
      721 atcccaaata accaaaggga aagcttggca ttccggtact gttggtaaag ccaccatgga
      781 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc
      841 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt
      901 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg
      961 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag
     1021 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt
     1081 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca
     1141 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa
     1201 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca
     1261 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt
     1321 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac
     1381 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc
     1441 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc
     1501 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt
     1561 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat
     1621 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga
     1681 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga
     1741 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct
     1801 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt
     1861 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct
     1921 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt
     1981 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga
     2041 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat
     2101 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc
     2161 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc
     2221 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt
     2281 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt
     2341 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc
     2401 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc
     2461 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     2521 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     2581 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     2641 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc
     2701 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga
     2761 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg
     2821 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     2881 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     2941 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     3001 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     3061 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     3121 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     3181 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     3241 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     3301 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     3361 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     3421 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     3481 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     3541 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     3601 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     3661 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     3721 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     3781 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     3841 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     3901 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
     3961 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     4021 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     4081 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     4141 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     4201 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     4261 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     4321 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     4381 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     4441 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     4501 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     4561 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     4621 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     4681 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     4741 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4801 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4861 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4921 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4981 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     5041 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     5101 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     5161 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat
     5221 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5281 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg
     5341 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga
     5401 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg
     5461 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata
//
