LOCUS       sequence-140846-        1066 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid driven under neuronal beta-tubulin promoter, bearing attB 
            and two insulator sequences (opposite directions pointing inward 
            towards the reporter gene).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Wang C, Szaro BG
  TITLE     A method for using direct injection of plasmid DNA to study 
            cis-regulatory element activity in F0 Xenopus embryos and tadpoles.
  JOURNAL   Dev Biol. 2015 Feb 1;398(1):11-23. doi: 10.1016/j.ydbio.2014.11.010.
            Epub 2014 Nov 20.
  PUBMED    25448690
REFERENCE   2  (bases 1 to 1066)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.ydbio.2014.11.010"; journalName: "Dev Biol"; date: 
            "2015-02-1- 1"; volume: "398"; issue: "1"; pages: "11-23"
COMMENT     Sequence Label: pSPORT1[attB/Ins1/NbT promoter/glGFP/bglobin
            3...
FEATURES             Location/Qualifiers
     source          1..1066
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(95..114)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    160..215
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(214..233)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
ORIGIN
        1 attttggcaa agaattcact cctcaggtgc aggctgccta tcagaaggtg gtggctggtg
       61 tggccaatgc cctggctcac aaacaccact gagatctttt tccctctgcc aaaaattatg
      121 gggacatcat gaagcccctt gagcatctga cttctggcta ataaaggaaa tttattttca
      181 ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg aaggacatat gggagggcaa
      241 atcatttaaa acatcagaat gagtatttgg tttagagttt ggcaacatat gcccatatgc
      301 tggctgccat gaacaaaggt tggctataaa gaggtcatca gtatatgaaa cagccccctg
      361 ctgtccattc cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt
      421 gttttgtgtt atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca
      481 gatttttcct cctctcctga ctactcccag tcatagctgt ccctcttctc ttatggagat
      541 ccctcgaggc ctcgagggat cttattaaag cagctgggac agggacagaa aaagggcttt
      601 gactgccttt ctcttgagcc cttttcctga tctccacaac tcactgatac cactggtctc
      661 attggaaggg gtgggctgtt aacagtgtga caaatgtagg aataaactgg atgcaaaagg
      721 gggctttgtg cagctttata ttcactgttg tcttaaaccc tttttatgga ctcaaatcaa
      781 atgacagtcc ctcaggatgt tagcttctga attcagaaag tgattgcaga gtttcccact
      841 cctttatcct gtgtctgatg gttttgctgt ctctgtagtg attagcttac tgtcaccatt
      901 tcctcattca ataggcactc aggtggatga aaggttctgg ttcactcccc aaatacctgc
      961 aacagtcagg agtgtgtcag gccaaaacca gaaaacagga attgccatgg ggtctccatg
     1021 atgggtggca gggactcaag tacatgagcc atattcggct gcttcc
//
