LOCUS       sequence-140913-        1476 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1476)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: PG1424PGA
FEATURES             Location/Qualifiers
     source          1..1476
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..21
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             46..66
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             1442..1459
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgcaccatc atcatcatca ttcttctggt gtagatctgg gtaccgagaa cctgtacttc
       61 caatccatga atccccctgc aggtcctgtg cgtgctatcg ctgagtacga acgctctgca
      121 gccgttttgg tacgctaccc gttcggtatc ccgatggaat tgatcaaaga gctggccaag
      181 aacgacaagg tgattaccat tgtggcgagt gaaagccaaa aaaacaccgt tataacccag
      241 tacacccaaa gcggtgtgaa tctctctaat tgcgatttca tcattgcgaa aactgactct
      301 tactggacac gcgactatac cggttggttc gcaatgtacg atacgaacaa agtaggtctc
      361 gtggacttta tttataaccg ccctcgtcct aacgatgatg aattccccaa atacgaagca
      421 caatatctgg gcatcgagat gttcgggatg aagctcaagc agaccggtgg caactacatg
      481 acggacggat atggatccgc tgtgcagtca catatcgcat atacggagaa ctcctctctg
      541 tctcaagctc aagtaaatca aaagatgaaa gactatctcg gcatcacaca tcatgatgtg
      601 gtacaagatc cgaacggcga atatatcaac catgtggact gttggggcaa gtatttggca
      661 ccgaacaaaa tcctcatcag gaaagtgcct gacaatcacc ctcagcacca agccctggaa
      721 gatatggcag cctacttcgc agcacagacc tgcgcatggg gaacgaagta cgaggtatat
      781 cgcgctttgg ccaccaatga acaaccgtac acgaactctc tgattctgaa caacagggta
      841 tttgttcctg tcaatggccc cgcctccgtg gacaacgatg ctctgaacgt ctataagacg
      901 gcaatgcccg gttacgaaat tataggtgtc aaaggggctt caggaacacc ttggttagga
      961 acagatgccc tgcattgtcg tactcacgag gtagcggata agggctatct ctatatcaag
     1021 cactacccga tactgggcga acaggcaggc cctgattata agatcgaagc agatgtcgtc
     1081 tcatgcgcca atgctactat ctcgccggta caatgttact atcgtatcaa tggttccggt
     1141 agctttaagg ctgctgatat gacgatggaa tcaacaggtc actatactta tagctttaca
     1201 ggtcttaaca agaatgataa ggtagaatac tatatctctg ccgctgacaa tagtggtcgc
     1261 aaagagactt atccctttat cggcgaacct gatcctttca agtttacgtg tatgaacgaa
     1321 accaatacat gtactgtgac cggagctgcc aaagctcttc gtgcatggtt caacgctgac
     1381 agtaaaggtg gatacggatc cgaattcgag ctccgtcgac aagcttgcgg ccgcactcga
     1441 gcaccaccac caccaccact gagatccggc tgctaa
//
