LOCUS       sequence-141012-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 RNA polymerase PCR cloned from BL21 (DE3) genomic DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-T7RNAP
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(22..65)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    68..87
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
ORIGIN
        1 ataggcgtat cacgaggcag aatttcagat aaaaaaaatc cttagctttc gctaaggatg
       61 atttctggaa ttcgcggccg cttctagaat ctagagacgc ataggtctca catatgaaca
      121 cgattaacat cgctaagaac gacttctctg acatcgaact ggctgctatc ccgttcaaca
      181 ctctggctga ccattacggt gagcgtttag ctcgcgaaca gttggccctt gagcatgagt
      241 cttacgagat gggtgaagca cgcttccgca agatgtttga gcgtcaactt aaagctggtg
      301 aggttgcgga taacgctgcc gccaagcctc tcatcactac cctactccct aagatgattg
      361 cacgcatcaa cgactggttt gaggaagtga aagctaagcg cggcaagcgc ccgacagcct
      421 tccagttcct gcaagaaatc aagccggaag ccgtagcgta catcaccatt aagaccactc
      481 tggcttgcct aaccagtgct gacaatacaa ccgttcaggc tgtagcaagc gcaatcggtc
      541 gggccattga ggacgaggct cgcttcggtc gtatccgtga ccttgaagct aagcacttca
      601 agaaaaacgt tgaggaacaa ctcaacaagc gcgtagggca cgtctacaag aaagcattta
      661 tgcaagttgt cgaggctgac atgctctcta agggtctact cggtggcgag gcgtggtctt
      721 cgtggcataa ggaagactct attcatgtag gagtacgctg catcgagatg ctcattgagt
      781 caaccggaat ggttagctta caccgccaa
//
