LOCUS       sequence-141054-        9156 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of DI-2A (hCD73/F2A/hCD39).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9156)
  AUTHORS   De Giorgi M, Pelikant-Malecka I, Sielicka A, Slominska EM, 
            Giovannoni R, Cinti A, Cerrito MG, Lavitrano M, Smolenski RT
  TITLE     Functional analysis of expression of human ecto-nucleoside 
            triphosphate diphosphohydrolase-1 and/or ecto-5'-nucleotidase in pig
            endothelial cells.
  JOURNAL   Nucleosides Nucleotides Nucleic Acids. 2014;33(4-6):313-8. doi: 
            10.1080/15257770.2014.896466.
  PUBMED    24940685
REFERENCE   2  (bases 1 to 9156)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleosides
            Nucleotides Nucleic Acids."; date: "2014"; volume: "33(4-6):313-8. 
            doi"; pages: " 10.1080/15257770.2014.896466"
COMMENT     Sequence Label: pCX-DI-2A
FEATURES             Location/Qualifiers
     source          1..9156
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        15..394
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        397..672
                     /label=chicken beta-actin promoter
     intron          673..1681
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1605..1627
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             3472..3537
                     /codon_start=1
                     /product="2A peptide from foot-and-mouth disease virus 
                     polyprotein"
                     /label=F2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="VKQTLNFDLLKLAGDVESNPGP"
     primer_bind     complement(5169..5188)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5234..5289
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5288..5307)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5648..5664)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5648..5664)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5661..5683)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5672..5688
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5696..5702,5703..5720,5721..5726))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5741..5762
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     polyA_signal    5944..6065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5981..6000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6035..6054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      complement(6072..6527)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6209..6230)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     6421..6440
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        6554..6658
                     /gene="bla"
                     /label=AmpR promoter
     promoter        6660..7017
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        6660..6851
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(6683..6703)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      6868..7003
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             7052..7846
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             7052..7846
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(7106..7125)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     7716..7735
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(8034..8053)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    8078..8125
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8454..9042
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8943..8962
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tagttattat ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
       61 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
      121 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
      181 aacgccaata gggactttcc attgacgtca atgggtggac tatttacggt aaactgccca
      241 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
      301 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
      361 gtacatctac gtattagtca tcgctattac catgggtcga ggtgagcccc acgttctgct
      421 tcactctccc catctccccc ccctccccac ccccaatttt gtatttattt attttttaat
      481 tattttgtgc agcgatgggg gcgggggggg ggggggcgcg cgccaggcgg ggcggggcgg
      541 ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg
      601 ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc
      661 gcgcggcggg cgggagtcgc tgcgttgcct tcgccccgtg ccccgctccg cgccgcctcg
      721 cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg gcgggacggc
      781 ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggctcgtttc ttttctgtgg
      841 ctgcgtgaaa gccttaaagg gctccgggag ggccctttgt gcggggggga gcggctcggg
      901 gggtgcgtgc gtgtgtgtgt gcgtggggag cgccgcgtgc ggcccgcgct gcccggcggc
      961 tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc gcgtgtgcgc gaggggagcg
     1021 cggccggggg cggtgccccg cggtgcgggg gggctgcgag gggaacaaag gctgcgtgcg
     1081 gggtgtgtgc gtgggggggt gagcaggggg tgtgggcgcg gcggtcgggc tgtaaccccc
     1141 ccctgcaccc ccctccccga gttgctgagc acggcccggc ttcgggtgcg gggctccgtg
     1201 cggggcgtgg cgcggggctc gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg
     1261 gcggggcggg gccgcctcgg gccggggagg gctcggggga ggggcgcggc ggccccggag
     1321 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga
     1381 gagggcgcag ggacttcctt tgtcccaaat ctggcggagc cgaaatctgg gaggcgccgc
     1441 cgcaccccct ctagcgggcg cgggcgaagc ggtgcggcgc cggcaggaag gaaatgggcg
     1501 gggagggcct tcgtgcgtcg ccgcgccgcc gtccccttct ccatctccag cctcggggct
     1561 gccgcagggg gacggctgcc ttcggggggg acggggcagg gcggggttcg gcttctggcg
     1621 tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct ttttcctaca
     1681 gctcctgggc aacgtgctgg ttgttgtgct gtctcatcat tttggcaaag aattcccacc
     1741 atgtgtcccc gagccgcgcg ggcgcccgcg acgctactcc tcgccctggg cgcggtgctg
     1801 tggcctgcgg ctggcgcctg ggagcttacg attttgcaca ccaacgacgt gcacagccgg
     1861 ctggagcaga ccagcgagga ctccagcaag tgcgtcaacg ccagccgctg catgggtggc
     1921 gtggctcggc tcttcaccaa ggttcagcag atccgccgcg ccgaacccaa cgtgctgctg
     1981 ctggacgccg gcgaccagta ccagggcact atctggttca ccgtgtacaa gggcgccgag
     2041 gtggcgcact tcatgaacgc cctgcgctac gatgccatgg cactgggaaa tcatgaattt
     2101 gataatggtg tggaaggact gatcgagcca ctcctcaaag aggccaaatt tccaattctg
     2161 agtgcaaaca ttaaagcaaa ggggccacta gcatctcaaa tatcaggact ttatttgcca
     2221 tataaagttc ttcctgttgg tgatgaagtt gtgggaatcg ttggatacac ttccaaagaa
     2281 accccttttc tctcaaatcc agggacaaat ttagtgtttg aagatgaaat cactgcatta
     2341 caacctgaag tagataagtt aaaaactcta aatgtgaaca aaattattgc actgggacat
     2401 tcgggttttg aaatggataa actcatcgct cagaaagtga ggggtgtgga cgtcgtggtg
     2461 ggaggacact ccaacacatt tctttacaca ggcaatccac cttccaaaga ggtgcctgct
     2521 gggaagtacc cattcatagt cacttctgat gatgggcgga aggttcctgt agtccaggcc
     2581 tatgcttttg gcaaatacct aggctatctg aagatcgagt ttgatgaaag aggaaacgtc
     2641 atctcttccc atggaaatcc cattcttcta aacagcagca ttcctgaaga tccaagcata
     2701 aaagcagaca ttaacaaatg gaggataaaa ttggataatt attctaccca ggaattaggg
     2761 aaaacaattg tctatctgga tggctcctct caatcatgcc gctttagaga atgcaacatg
     2821 ggcaacctga tttgtgatgc aatgattaac aacaacctga gacacacgga tgaaatgttc
     2881 tggaaccacg tatccatgtg cattttaaat ggaggtggta tccggtcgcc cattgatgaa
     2941 cgcaacaatg gcacaattac ctgggagaac ctggctgctg tattgccctt tggaggcaca
     3001 tttgacctag tccagttaaa aggttccacc ctgaagaagg cctttgagca tagcgtgcac
     3061 cgctacggcc agtccactgg agagttcctg caggtgggcg gaatccatgt ggtgtatgat
     3121 ctttcccgaa aacctggaga cagagtagtc aaattagatg ttctttgcac caagtgtcga
     3181 gtgcccagtt atgaccctct caaaatggac gaggtatata aggtgatcct cccaaacttc
     3241 ctggccaatg gtggagatgg gttccagatg ataaaagatg aattattaag acatgactct
     3301 ggtgaccaag atatcaacgt ggtttctaca tatatctcca aaatgaaagt aatttatcca
     3361 gcagttgaag gtcggatcaa gttttccaca ggaagtcact gccatggaag cttttcttta
     3421 atatttcttt cactttgggc agtgatcttt gttttatacc aatagggatc cgtgaaacag
     3481 actttgaatt ttgaccttct caagttggcg ggagacgtgg agtccaaccc agggcccggc
     3541 agcggcctcg agatggaaga tacaaaggag tctaacgtga agacattttg ctccaagaat
     3601 atcctagcca tccttggctt ctcctctatc atagctgtga tagctttgct tgctgtgggg
     3661 ttgacccaga acaaagcatt gccagaaaac gttaagtatg ggattgtgct ggatgcgggt
     3721 tcttctcaca caagtttata catctataag tggccagcag aaaaggagaa tgacacaggc
     3781 gtggtgcatc aagtagaaga atgcagggtt aaaggtcctg gaatctcaaa atttgttcag
     3841 aaagtaaatg aaataggcat ttacctgact gattgcatgg aaagagctag ggaagtgatt
     3901 ccaaggtccc agcaccaaga gacacccgtt tacctgggag ccacggcagg catgcggttg
     3961 ctcaggatgg aaagtgaaga gttggcagac agggttctgg atgtggtgga gaggagcctc
     4021 agcaactacc cctttgactt ccagggtgcc aggatcatta ctggccaaga ggaaggtgcc
     4081 tatggctgga ttactatcaa ctatctgctg ggcaaattca gtcagaaaac aaggtggttc
     4141 agcatagtcc catatgaaac caataatcag gaaacctttg gagctttgga ccttggggga
     4201 gcctctacac aagtcacttt tgtaccccaa aaccagacta tcgagtcccc agataatgct
     4261 ctgcaatttc gcctctatgg caaggactac aatgtctaca cacatagctt cttgtgctat
     4321 gggaaggatc aggcactctg gcagaaactg gccaaggaca ttcaggttgc aagtaatgaa
     4381 attctcaggg acccatgctt tcatcctgga tataagaagg tagtgaacgt aagtgacctt
     4441 tacaagaccc cctgcaccaa gagatttgag atgactcttc cattccagca gtttgaaatc
     4501 cagggtattg gaaactatca acaatgccat caaagcatcc tggagctctt caacaccagt
     4561 tactgccctt actcccagtg tgccttcaat gggattttct tgccaccact ccagggggat
     4621 tttggggcat tttcagcttt ttactttgtg atgaagtttt taaacttgac atcagagaaa
     4681 gtctctcagg aaaaggtgac tgagatgatg aaaaagttct gtgctcagcc ttgggaggag
     4741 ataaaaacat cttacgctgg agtaaaggag aagtacctga gtgaatactg cttttctggt
     4801 acctacattc tctccctcct tctgcaaggc tatcatttca cagctgattc ctgggagcac
     4861 atccatttca ttggcaagat ccagggcagc gacgccggct ggactttggg ctacatgctg
     4921 aacctgacca acatgatccc agctgagcaa ccattgtcca cacctctctc ccactccacc
     4981 tatgtcttcc tcatggttct attctccctg gtccttttca cagtggccat cataggcttg
     5041 cttatctttc acaagccttc atatttctgg aaagatatgg tataggaatt cactcctcag
     5101 gtgcaggctg cctatcagaa ggtggtggct ggtgtggcca atgccctggc tcacaaatac
     5161 cactgagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat
     5221 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg
     5281 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat
     5341 ttggtttaga gtttggcaac atatgccata tgctggctgc catgaacaaa ggtggctata
     5401 aagaggtcat cagtatatga aacagccccc tgctgtccat tccttattcc atagaaaagc
     5461 cttgacttga ggttagattt tttttatatt ttgttttgtg ttattttttt ctttaacatc
     5521 cctaaaattt tccttacatg ttttactagc cagatttttc ctcctctcct gactactccc
     5581 agtcatagct gtccctcttc tcttatgaag atccctcgac ctgcagccca agcttggcgt
     5641 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     5701 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     5761 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc
     5821 accggatcta gataactgat cataatcagc cataccacat ttgtagaggt tttacttgct
     5881 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     5941 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6061 tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta
     6121 aatcagctca ttttttaacc aataggccga aatcggcaaa atcccttata aatcaaaaga
     6181 atagaccgag atagggttga gtgttgttcc agtttggaac aagagtccac tattaaagaa
     6241 cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga
     6301 accatcaccc taatcaagtt ttttggggtc gaggtgccgt aaagcactaa atcggaaccc
     6361 taaagggagc ccccgattta gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga
     6421 agggaagaaa gcgaaaggag cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg
     6481 cgtaaccacc acacccgccg cgcttaatgc gccgctacag ggcgcgtcag gtggcacttt
     6541 tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta
     6601 tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtcc
     6661 tgaggcggaa agaaccagct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     6721 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     6781 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     6841 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6901 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6961 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaagat
     7021 cgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
     7081 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
     7141 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
     7201 gaccgacctg tccggtgccc tgaatgaact gcaagacgag gcagcgcggc tatcgtggct
     7261 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
     7321 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
     7381 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
     7441 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
     7501 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
     7561 gttcgccagg ctcaaggcga gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
     7621 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
     7681 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
     7741 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
     7801 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
     7861 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
     7921 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
     7981 cagcgcgggg atctcatgct ggagttcttc gcccacccta gggggaggct aactgaaaca
     8041 cggaaggaga caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa
     8101 acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc ggtcccaggg ctggcactct
     8161 gtcgataccc caccgagacc ccattggggc caatacgccc gcgtttcttc cttttcccca
     8221 ccccaccccc caagttcggg tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc
     8281 cctgccatag cctcaggtta ctcatatata ctttagattg atttaaaact tcatttttaa
     8341 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     8401 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     8461 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     8521 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     8581 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     8641 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     8701 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     8761 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8821 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8881 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8941 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     9001 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     9061 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     9121 cctgattctg tggataaccg tattaccgcc atgcat
//
